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Innate immune homeostasis by the homeobox gene Caudal and commensal-gut mutualism in Drosophila

Authors
 Ji-Hwan Ryu  ;  Sung-Hee Kim  ;  Hyo-Young Lee  ;  Jin Young Bai  ;  Young-Do Nam  ;  Jin-Woo Bae  ;  Dong Gun Lee  ;  Seung Chul Shin  ;  Eun-Mi Ha  ;  Won-Jae Lee 
Citation
 SCIENCE, Vol.319(5864) : 777-782, 2008 
Journal Title
 SCIENCE 
ISSN
 0036-8075 
Issue Date
2008
MeSH
Acetobacteraceae/growth & development ; Animals ; Animals, Genetically Modified ; Antibiosis ; Antimicrobial Cationic Peptides/biosynthesis ; Antimicrobial Cationic Peptides/genetics ; Apoptosis ; Bacteria/growth & development ; Drosophila Proteins/genetics* ; Drosophila Proteins/metabolism ; Drosophila Proteins/physiology ; Drosophila melanogaster/genetics ; Drosophila melanogaster/immunology* ; Drosophila melanogaster/metabolism ; Drosophila melanogaster/microbiology* ; Epithelial Cells/cytology ; Epithelial Cells/metabolism ; Gene Expression Regulation ; Genes, Homeobox* ; Genes, Insect ; Germ-Free Life ; Gluconobacter/growth & development* ; Gluconobacter/pathogenicity ; Homeodomain Proteins/genetics* ; Homeodomain Proteins/physiology ; Homeostasis ; Immunity, Innate* ; Intestinal Mucosa/metabolism ; Intestines/cytology ; Intestines/immunology ; Intestines/microbiology ; RNA Interference ; Symbiosis ; Transcription Factors/genetics* ; Transcription Factors/metabolism ; Transcription Factors/physiology
Keywords
Acetobacteraceae/growth & development ; Animals ; Animals, Genetically Modified ; Antibiosis ; Antimicrobial Cationic Peptides/biosynthesis ; Antimicrobial Cationic Peptides/genetics ; Apoptosis ; Bacteria/growth & development ; Drosophila Proteins/genetics* ; Drosophila Proteins/metabolism ; Drosophila Proteins/physiology ; Drosophila melanogaster/genetics ; Drosophila melanogaster/immunology* ; Drosophila melanogaster/metabolism ; Drosophila melanogaster/microbiology* ; Epithelial Cells/cytology ; Epithelial Cells/metabolism ; Gene Expression Regulation ; Genes, Homeobox* ; Genes, Insect ; Germ-Free Life ; Gluconobacter/growth & development* ; Gluconobacter/pathogenicity ; Homeodomain Proteins/genetics* ; Homeodomain Proteins/physiology ; Homeostasis ; Immunity, Innate* ; Intestinal Mucosa/metabolism ; Intestines/cytology ; Intestines/immunology ; Intestines/microbiology ; RNA Interference ; Symbiosis ; Transcription Factors/genetics* ; Transcription Factors/metabolism ; Transcription Factors/physiology
Abstract
Although commensalism with gut microbiota exists in all metazoans, the host factors that maintain this homeostatic relationship remain largely unknown. We show that the intestinal homeobox gene Caudal regulates the commensal-gut mutualism by repressing nuclear factor kappa B-dependent antimicrobial peptide genes. Inhibition of Caudal expression in flies via RNA interference led to overexpression of antimicrobial peptides, which in turn altered the commensal population within the intestine. In particular, the dominance of one gut microbe, Gluconobacter sp. strain EW707, eventually led to gut cell apoptosis and host mortality. However, restoration of a healthy microbiota community and normal host survival in the Caudal-RNAi flies was achieved by reintroduction of the Caudal gene. These results reveal that a specific genetic deficiency within a host can profoundly influence the gut commensal microbial community and host physiology
Files in This Item:
T200805830.pdf Download
DOI
10.1126/science.1149357
Appears in Collections:
5. Research Institutes (연구소) > Research Center for Human Natural Defense System (생체방어연구센터) > 1. Journal Papers
Yonsei Authors
Ryu, Ji Hwan(유지환)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/108551
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