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Decreased potency of the Vibrio cholerae sheathed flagellum to trigger host innate immunity.

Authors
 Sang Sun Yoon  ;  John J. Mekalanos 
Citation
 INFECTION AND IMMUNITY, Vol.76(3) : 1282-1288, 2008 
Journal Title
INFECTION AND IMMUNITY
ISSN
 0019-9567 
Issue Date
2008
MeSH
Bacterial Proteins/immunology ; Cell Line ; Epithelial Cells/immunology ; Epithelial Cells/microbiology ; Flagella/immunology* ; Flagellin/immunology ; Flagellin/isolation & purification ; Humans ; Immunity, Innate* ; NF-kappa B/metabolism ; Salmonella typhimurium/immunology ; Vibrio cholerae/immunology*
Keywords
Bacterial Proteins/immunology ; Cell Line ; Epithelial Cells/immunology ; Epithelial Cells/microbiology ; Flagella/immunology* ; Flagellin/immunology ; Flagellin/isolation & purification ; Humans ; Immunity, Innate* ; NF-kappa B/metabolism ; Salmonella typhimurium/immunology ; Vibrio cholerae/immunology*
Abstract
Vibrio cholerae is a monoflagellated gram-negative bacterium that causes the severe diarrheal disease cholera. In contrast to Salmonella enterica serovar Typhimurium infection, which is accompanied by both acute diarrhea and high-level inflammation, V. cholerae infection is largely noninflammatory in human hosts. Bacterial flagella are composed of flagellin, a highly conserved protein that is also a target of the innate immune response. Because the V. cholerae flagellum is covered by a sheath, we hypothesized that it might be less prone to activation of the innate immune response than nonsheathed flagella, such as those produced by Salmonella serovar Typhimurium. Indeed, compared with Salmonella serovar Typhimurium flagella, V. cholerae flagella demonstrated significantly reduced NF-kappaB activation in A549 human pulmonary epithelial cells. However, V. cholerae flagellin monomers, FlaD and FlaC, were almost equally potent with purified FliC, a monomer derived from Salmonella serovar Typhimurium flagella, in NF-kappaB activation. Heat- and acid-induced dissociation assays showed that Salmonella serovar Typhimurium flagella disassembled far more readily into monomeric flagellins than V. cholerae flagella, suggesting that the differential levels of NF-kappaB activation by V. cholerae and Salmonella serovar Typhimurium flagella are likely attributable to the difference in their flagellin shedding. Our results suggest that monomer dissociation of V. cholerae flagella is suppressed likely due to the presence of the sheath and that this unique structural feature of V. cholerae flagella may have evolved as a strategy to evade flagellin-triggered host innate immune responses in various host species.
Files in This Item:
T200805826.pdf Download
DOI
10.1128/IAI.00736-07
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Yoon, Sang Sun(윤상선) ORCID logo https://orcid.org/0000-0003-2979-365X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/108548
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