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Mutation in the transcriptional regulator PhoP contributes to avirulence of Mycobacterium tuberculosis H37Ra strain.

DC Field Value Language
dc.contributor.author전보영-
dc.contributor.author조상래-
dc.contributor.author곽정연-
dc.date.accessioned2015-05-19T17:27:36Z-
dc.date.available2015-05-19T17:27:36Z-
dc.date.issued2008-
dc.identifier.issn1931-3128-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/108188-
dc.description.abstractAttenuated strains of mycobacteria can be exploited to determine genes essential for their pathogenesis and persistence. To this goal, we sequenced the genome of H37Ra, an attenuated variant of Mycobacterium tuberculosis H37Rv strain. Comparison with H37Rv revealed three unique coding region polymorphisms. One polymorphism was located in the DNA-binding domain of the transcriptional regulator PhoP, causing the protein's diminished DNA-binding capacity. Temporal gene expression profiles showed that several genes with reduced expression in H37Ra were also repressed in an H37Rv phoP knockout strain. At later time points, genes of the dormancy regulon, typically expressed in a state of nonreplicating persistence, were upregulated in H37Ra. Complementation of H37Ra with H37Rv phoP partially restored its persistence in a murine macrophage infection model. Our approach demonstrates the feasibility of identifying minute but distinct differences between isogenic strains and illustrates the consequences of single point mutations on the survival stratagem of M. tuberculosis-
dc.description.statementOfResponsibilityopen-
dc.format.extent97~103-
dc.relation.isPartOfCELL HOST & MICROBE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAmino Acid Sequence-
dc.subject.MESHAnimals-
dc.subject.MESHBacterial Proteins/chemistry-
dc.subject.MESHBacterial Proteins/genetics*-
dc.subject.MESHCells, Cultured-
dc.subject.MESHGenetic Complementation Test-
dc.subject.MESHMacrophages/microbiology-
dc.subject.MESHMice-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHMycobacterium tuberculosis/chemistry*-
dc.subject.MESHMycobacterium tuberculosis/pathogenicity*-
dc.subject.MESHPoint Mutation-
dc.subject.MESHPolymorphism, Genetic-
dc.subject.MESHProtein Structure, Tertiary/genetics-
dc.subject.MESHSequence Alignment-
dc.subject.MESHTranscription Factors/chemistry-
dc.subject.MESHTranscription Factors/genetics*-
dc.subject.MESHVirulence-
dc.titleMutation in the transcriptional regulator PhoP contributes to avirulence of Mycobacterium tuberculosis H37Ra strain.-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Microbiology (미생물학)-
dc.contributor.googleauthorJong Seok Lee-
dc.contributor.googleauthorRoland Krause-
dc.contributor.googleauthorJorg Schreiber-
dc.contributor.googleauthorHans-Joachim Mollenkopf-
dc.contributor.googleauthorJane Kowall-
dc.contributor.googleauthorRobert Stein-
dc.contributor.googleauthorBo-Young Jeon-
dc.contributor.googleauthorJeong-Yeon Kwak-
dc.contributor.googleauthorMin-Kyong Song-
dc.contributor.googleauthorJuan Pablo Patron-
dc.contributor.googleauthorSabine Jorg-
dc.contributor.googleauthorKyoungmin Roh-
dc.contributor.googleauthorSang-Nae Cho-
dc.contributor.googleauthorStefan H.E. Kaufmann-
dc.identifier.doi10.1016/j.chom.2008.01.002-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA03824-
dc.contributor.localIdA00180-
dc.contributor.localIdA03516-
dc.relation.journalcodeJ00484-
dc.identifier.eissn1934-6069-
dc.identifier.pmid18312844-
dc.subject.keywordAmino Acid Sequence-
dc.subject.keywordAnimals-
dc.subject.keywordBacterial Proteins/chemistry-
dc.subject.keywordBacterial Proteins/genetics*-
dc.subject.keywordCells, Cultured-
dc.subject.keywordGenetic Complementation Test-
dc.subject.keywordMacrophages/microbiology-
dc.subject.keywordMice-
dc.subject.keywordMolecular Sequence Data-
dc.subject.keywordMycobacterium tuberculosis/chemistry*-
dc.subject.keywordMycobacterium tuberculosis/pathogenicity*-
dc.subject.keywordPoint Mutation-
dc.subject.keywordPolymorphism, Genetic-
dc.subject.keywordProtein Structure, Tertiary/genetics-
dc.subject.keywordSequence Alignment-
dc.subject.keywordTranscription Factors/chemistry-
dc.subject.keywordTranscription Factors/genetics*-
dc.subject.keywordVirulence-
dc.contributor.alternativeNameJeon, Bo Young-
dc.contributor.alternativeNameCho, Sang Nae-
dc.contributor.alternativeNameKwak, Jeong Yeon-
dc.contributor.affiliatedAuthorCho, Sang Nae-
dc.contributor.affiliatedAuthorKwak, Jeong Yeon-
dc.contributor.affiliatedAuthorJeon, Bo Young-
dc.rights.accessRightsfree-
dc.citation.volume3-
dc.citation.number2-
dc.citation.startPage97-
dc.citation.endPage103-
dc.identifier.bibliographicCitationCELL HOST & MICROBE, Vol.3(2) : 97-103, 2008-
dc.identifier.rimsid35237-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers

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