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Mite and cockroach allergens activate protease-activated receptor 2 and delay epidermal permeability barrier recovery

Authors
 Se Kyoo Jeong  ;  Hyun Jeong Kim  ;  Jong-Kyung Youm  ;  Sung Ku Ahn  ;  EungHo Choi  ;  Myung Hyun Sohn  ;  Kyu-Earn Kim  ;  Jeong Hee Hong  ;  Dong Min Shin  ;  Seung Hun Lee 
Citation
 JOURNAL OF INVESTIGATIVE DERMATOLOGY, Vol.128(8) : 1930-1939, 2008 
Journal Title
 JOURNAL OF INVESTIGATIVE DERMATOLOGY 
ISSN
 0022-202X 
Issue Date
2008
MeSH
Adult ; Animals ; Antigens, Dermatophagoides/immunology* ; Biopsy ; Calcium/metabolism ; Cell Membrane Permeability/immunology* ; Cell Membrane Permeability/physiology ; Cells, Cultured ; Cockroaches ; Dermatitis, Atopic/etiology ; Dermatitis, Atopic/immunology* ; Dermatitis, Atopic/pathology ; Epidermis/immunology* ; Epidermis/metabolism ; Epidermis/pathology ; Female ; Homeostasis/immunology ; Homeostasis/physiology ; Humans ; Keratinocytes/immunology* ; Keratinocytes/metabolism ; Keratinocytes/pathology ; Male ; Mice ; Mice, Hairless ; Receptor, PAR-2/antagonists & inhibitors ; Receptor, PAR-2/metabolism* ; Signal Transduction/immunology ; Signal Transduction/physiology
Keywords
Adult ; Animals ; Antigens, Dermatophagoides/immunology* ; Biopsy ; Calcium/metabolism ; Cell Membrane Permeability/immunology* ; Cell Membrane Permeability/physiology ; Cells, Cultured ; Cockroaches ; Dermatitis, Atopic/etiology ; Dermatitis, Atopic/immunology* ; Dermatitis, Atopic/pathology ; Epidermis/immunology* ; Epidermis/metabolism ; Epidermis/pathology ; Female ; Homeostasis/immunology ; Homeostasis/physiology ; Humans ; Keratinocytes/immunology* ; Keratinocytes/metabolism ; Keratinocytes/pathology ; Male ; Mice ; Mice, Hairless ; Receptor, PAR-2/antagonists & inhibitors ; Receptor, PAR-2/metabolism* ; Signal Transduction/immunology ; Signal Transduction/physiology
Abstract
Protease-activated receptor-2 (PAR-2) is known to be involved in epidermal permeability barrier function homeostasis. PAR-2 activation occurs after barrier disruption and further activation of PAR-2 by activating peptide significantly delays barrier recovery rate. Cockroach and house dust mite allergens, both known to be associated with the development of asthma, allergic rhinitis, and atopic dermatitis, have protease activity, which can activate PAR-2. In this study, we investigated the effects of both allergens on the epidermal barrier function as well as on the epidermal calcium gradient. Both allergens, when topically applied on the barrier-disrupted site, increased protease activities in the epidermis and delayed barrier recovery and lamellar body secretion in murine skin. The topical application of PAR-2-specific antagonist or protease inhibitors normalized the barrier recovery. Cockroach allergens induced intracellular calcium oscillations in cultured human keratinocytes through PAR-2-involved pathway, which was confirmed by desensitization protocol. Abnormal calcium ion distribution after barrier disruption was also observed in allergens-applied skin. These results suggest that allergens with protease activity can influence the epidermal permeability barrier homeostasis through PAR-2 activation and consequent modulation of the calcium ions in skin.
Files in This Item:
T200803294.pdf Download
DOI
10.1038/jid.2008.13
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyu Earn(김규언)
Kim, Hyun Jung(김현정)
Sohn, Myung Hyun(손명현) ORCID logo https://orcid.org/0000-0002-2478-487X
Shin, Dong Min(신동민) ORCID logo https://orcid.org/0000-0001-6042-0435
Lee, Seung Hun(이승헌)
Hong, Jeong Hee(홍정희)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/108097
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