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Effect of sodium hydroxide treatment of bacterial cellulose on cellulase activity

Authors
 Hyungil Jung  ;  Ho Geun Yoon  ;  Woojun Park  ;  Cheol Choi  ;  David B. Wilson  ;  Dong Hoon Shin  ;  Young Jun Kim 
Citation
 CELLULOSE, Vol.15(3) : 465-471, 2008 
Journal Title
 CELLULOSE 
ISSN
 0969-0239 
Issue Date
2008
Keywords
Cellulose ; Endocelluase ; Exocellulase ; NaOH ; Synergism ; Thermobifida fusca
Abstract
The synergistic action between Thermobifida fusca exocellulase Cel6B and endocellulase Cel5A on sodium hydroxide pretreated bacterial cellulose (BC) was determined. The activities of Cel6B and Cel5A were tested singly and both activities were dramatically increased on pretreated BC, especially in the early stage of hydrolysis. Cel5A, which attacks the cellulose chain randomly, showed a larger increase on NaOH treated BC than Cel6B. Mixtures of the two enzymes were also able to degrade NaOH treated BC faster than BC and the kinetics of the mixture differed from that of the individual enzymes. The degree of synergistic effect (DSE) on BC decreased dramatically with time of hydrolysis. However, the DSE on NaOH treated BC was almost constant throughout the incubation, with a smaller effect at higher NaOH concentrations. The change caused by NaOH did not increase the DSE, although each individual cellulase activity increased. This showed that synergistic activity was more effective on recalcitrant cellulose, which requires effective cooperation between the cellulase components for hydrolysis
Full Text
http://link.springer.com/article/10.1007/s10570-007-9190-4#
DOI
10.1007/s10570-007-9190-4
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Yoon, Ho Geun(윤호근) ORCID logo https://orcid.org/0000-0003-2718-3372
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/108003
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