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Lipin1 is a key factor for the maturation and maintenance of adipocytes in the regulatory network with CCAAT/enhancer-binding protein alpha and peroxisome proliferator-activated receptor gamma 2.

 Yoo-Kyung Koh  ;  Min-Young Lee  ;  Jae-Woo Kim  ;  Minji Kim  ;  Jong-Seok Moon  ;  Yoo-Jung Lee  ;  Yong-Ho Ahn  ;  Kyung-Sup Kim 
 Journal of Biological Chemistry, Vol.283(50) : 34896-34906, 2008 
Journal Title
 Journal of Biological Chemistry 
Issue Date
Lipin1 expression was induced at a late stage of differentiation of 3T3-L1 preadipocytes and maintained at high levels in mature adipocytes. Knockdown of expression of lipin1 by small interfering RNA in 3T3-L1 preadipocytes almost completely inhibited differentiation into adipocytes, whereas overexpression of lipin1 accelerated adipocyte differentiation, demonstrating that lipin1 is required for adipocyte differentiation. In mature adipocytes, transfection of lipin1-small interfering RNA decreased the expression of adipocyte functional genes, indicating the involvement of lipin1 in the maintenance of adipocyte function. Lipin1 increases the transcription-activating function of peroxisome proliferator-activated receptor gamma(2) (PPAR gamma(2)) via direct physical interaction, whereas lipin1 did not affect the function of other adipocyte-related transcription factors such as C/EBP alpha, liver X-activated receptor alpha, or sterol regulatory element binding protein 1c. In mature adipocytes, lipin1 was specifically recruited to the PPAR gamma-response elements of the phosphoenolpyruvate carboxykinase gene, an adipocyte-specific gene. C/EBP alpha up-regulates lipin1 transcription by directly binding to the lipin1 promoter. Based on the existence of a positive feedback loop between C/EBP alpha and PPAR gamma(2), we propose that lipin1 functions as an amplifier of the network between these factors, resulting in the maintenance of high levels of the specific gene expression that are required for adipogenesis and mature adipocyte functions.
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1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
김경섭(Kim, Kyung Sup) ORCID logo https://orcid.org/0000-0001-8483-8537
김재우(Kim, Jae Woo) ORCID logo https://orcid.org/0000-0001-5456-9495
안용호(Ahn, Yong Ho) ORCID logo https://orcid.org/0000-0002-4133-0757
이민영(Lee, Min Young)
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