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Cited 84 times in

Lipin1 is a key factor for the maturation and maintenance of adipocytes in the regulatory network with CCAAT/enhancer-binding protein alpha and peroxisome proliferator-activated receptor gamma 2.

DC FieldValueLanguage
dc.contributor.author김경섭-
dc.contributor.author김재우-
dc.contributor.author안용호-
dc.contributor.author이민영-
dc.date.accessioned2015-05-19T17:20:27Z-
dc.date.available2015-05-19T17:20:27Z-
dc.date.issued2008-
dc.identifier.issn0021-9258-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/107962-
dc.description.abstractLipin1 expression was induced at a late stage of differentiation of 3T3-L1 preadipocytes and maintained at high levels in mature adipocytes. Knockdown of expression of lipin1 by small interfering RNA in 3T3-L1 preadipocytes almost completely inhibited differentiation into adipocytes, whereas overexpression of lipin1 accelerated adipocyte differentiation, demonstrating that lipin1 is required for adipocyte differentiation. In mature adipocytes, transfection of lipin1-small interfering RNA decreased the expression of adipocyte functional genes, indicating the involvement of lipin1 in the maintenance of adipocyte function. Lipin1 increases the transcription-activating function of peroxisome proliferator-activated receptor gamma(2) (PPAR gamma(2)) via direct physical interaction, whereas lipin1 did not affect the function of other adipocyte-related transcription factors such as C/EBP alpha, liver X-activated receptor alpha, or sterol regulatory element binding protein 1c. In mature adipocytes, lipin1 was specifically recruited to the PPAR gamma-response elements of the phosphoenolpyruvate carboxykinase gene, an adipocyte-specific gene. C/EBP alpha up-regulates lipin1 transcription by directly binding to the lipin1 promoter. Based on the existence of a positive feedback loop between C/EBP alpha and PPAR gamma(2), we propose that lipin1 functions as an amplifier of the network between these factors, resulting in the maintenance of high levels of the specific gene expression that are required for adipogenesis and mature adipocyte functions.-
dc.description.statementOfResponsibilityopen-
dc.format.extent34896~34906-
dc.relation.isPartOfJOURNAL OF BIOLOGICAL CHEMISTRY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESH3T3-L1 Cells-
dc.subject.MESHAdipocytes/metabolism-
dc.subject.MESHAnimals-
dc.subject.MESHCCAAT-Enhancer-Binding Protein-alpha/metabolism*-
dc.subject.MESHCell Differentiation-
dc.subject.MESHDNA-Binding Proteins/metabolism-
dc.subject.MESHLiver X Receptors-
dc.subject.MESHMice-
dc.subject.MESHModels, Biological-
dc.subject.MESHNIH 3T3 Cells-
dc.subject.MESHNuclear Proteins/chemistry*-
dc.subject.MESHOligonucleotides/chemistry-
dc.subject.MESHOrphan Nuclear Receptors-
dc.subject.MESHPPAR gamma/metabolism*-
dc.subject.MESHPhosphatidate Phosphatase-
dc.subject.MESHPlasmids/metabolism-
dc.subject.MESHRNA, Small Interfering/metabolism-
dc.subject.MESHReceptors, Cytoplasmic and Nuclear/metabolism-
dc.subject.MESHSterol Regulatory Element Binding Protein 1/metabolism-
dc.titleLipin1 is a key factor for the maturation and maintenance of adipocytes in the regulatory network with CCAAT/enhancer-binding protein alpha and peroxisome proliferator-activated receptor gamma 2.-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Biochemistry & Molecular Biology (생화학,분자생물학)-
dc.contributor.googleauthorYoo-Kyung Koh-
dc.contributor.googleauthorMin-Young Lee-
dc.contributor.googleauthorJae-Woo Kim-
dc.contributor.googleauthorMinji Kim-
dc.contributor.googleauthorJong-Seok Moon-
dc.contributor.googleauthorYoo-Jung Lee-
dc.contributor.googleauthorYong-Ho Ahn-
dc.contributor.googleauthorKyung-Sup Kim-
dc.identifier.doi10.1074/jbc.M804007200-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00297-
dc.contributor.localIdA00865-
dc.contributor.localIdA02249-
dc.contributor.localIdA02783-
dc.relation.journalcodeJ01258-
dc.identifier.eissn1083-351X-
dc.identifier.pmid18930917-
dc.subject.keyword3T3-L1 Cells-
dc.subject.keywordAdipocytes/metabolism-
dc.subject.keywordAnimals-
dc.subject.keywordCCAAT-Enhancer-Binding Protein-alpha/metabolism*-
dc.subject.keywordCell Differentiation-
dc.subject.keywordDNA-Binding Proteins/metabolism-
dc.subject.keywordLiver X Receptors-
dc.subject.keywordMice-
dc.subject.keywordModels, Biological-
dc.subject.keywordNIH 3T3 Cells-
dc.subject.keywordNuclear Proteins/chemistry*-
dc.subject.keywordOligonucleotides/chemistry-
dc.subject.keywordOrphan Nuclear Receptors-
dc.subject.keywordPPAR gamma/metabolism*-
dc.subject.keywordPhosphatidate Phosphatase-
dc.subject.keywordPlasmids/metabolism-
dc.subject.keywordRNA, Small Interfering/metabolism-
dc.subject.keywordReceptors, Cytoplasmic and Nuclear/metabolism-
dc.subject.keywordSterol Regulatory Element Binding Protein 1/metabolism-
dc.contributor.alternativeNameKim, Kyung Sup-
dc.contributor.alternativeNameKim, Jae Woo-
dc.contributor.alternativeNameAhn, Yong Ho-
dc.contributor.alternativeNameLee, Min Young-
dc.contributor.affiliatedAuthorKim, Kyung Sup-
dc.contributor.affiliatedAuthorKim, Jae Woo-
dc.contributor.affiliatedAuthorAhn, Yong Ho-
dc.contributor.affiliatedAuthorLee, Min Young-
dc.rights.accessRightsfree-
dc.citation.volume283-
dc.citation.number50-
dc.citation.startPage34896-
dc.citation.endPage34906-
dc.identifier.bibliographicCitationJOURNAL OF BIOLOGICAL CHEMISTRY, Vol.283(50) : 34896-34906, 2008-
dc.identifier.rimsid34812-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers

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