486 367

Cited 58 times in

Replicative senescence induced by Romo1-derived reactive oxygen species.

Authors
 Young Min Chung  ;  Seung Baek Lee  ;  Hyung Jung Kim  ;  Seon Ho Park  ;  Jung Jin Kim  ;  Jin Sil Chung  ;  Young Do Yoo 
Citation
 JOURNAL OF BIOLOGICAL CHEMISTRY, Vol.283(48) : 33763-33771, 2008 
Journal Title
JOURNAL OF BIOLOGICAL CHEMISTRY
ISSN
 0021-9258 
Issue Date
2008
MeSH
Cell Line ; Cellular Senescence/physiology* ; DNA Damage/physiology* ; Electron Transport Chain Complex Proteins/metabolism ; Fibroblasts/cytology ; Fibroblasts/metabolism* ; Gene Expression Regulation/physiology* ; Humans ; Membrane Proteins/biosynthesis* ; Membrane Proteins/genetics ; Mitochondrial Proteins/biosynthesis* ; Mitochondrial Proteins/genetics ; RNA, Small Interfering ; Reactive Oxygen Species/metabolism*
Keywords
Cell Line ; Cellular Senescence/physiology* ; DNA Damage/physiology* ; Electron Transport Chain Complex Proteins/metabolism ; Fibroblasts/cytology ; Fibroblasts/metabolism* ; Gene Expression Regulation/physiology* ; Humans ; Membrane Proteins/biosynthesis* ; Membrane Proteins/genetics ; Mitochondrial Proteins/biosynthesis* ; Mitochondrial Proteins/genetics ; RNA, Small Interfering ; Reactive Oxygen Species/metabolism*
Abstract
Persistent accumulation of DNA damage induced by reactive oxygen species (ROS) is proposed to be a major contributor toward the aging process. Furthermore, an increase in age-associated ROS is strongly correlated with aging in various species, including humans. Here we showed that the enforced expression of the ROS modulator 1 (Romo1) triggered premature senescence by ROS production, and this also contributed toward induction of DNA damage. Romo1-derived ROS was found to originate in the mitochondrial electron transport chain. Romo1 expression gradually increased in proportion to population doublings of IMR-90 human fibroblasts. An increase in ROS production in these cells with high population doubling was blocked by the Romo1 knockdown using Romo1 small interfering RNA. Romo1 knockdown also inhibited the progression of replicative senescence. Based on these results, we suggest that age-related ROS levels increase, and this contributes to replicative senescence, which is directly associated with Romo1 expression
Files in This Item:
T200801445.pdf Download
DOI
10.1074/jbc.M805334200
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyung Jung(김형중) ORCID logo https://orcid.org/0000-0003-2498-0683
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/107751
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links