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Multiplex mini Y short tandem repeat haplotyping using fluorescence energy transfer labeled primers

Authors
 Tae Seok Seo  ;  Kyoung-Jin Shin  ;  Jong Young Choi  ;  Richard A. Mathies 
Citation
 BIOCHIP JOURNAL, Vol.2(3) : 192-199, 2008 
Journal Title
 BIOCHIP JOURNAL 
ISSN
 1976-0280 
Issue Date
2008
Keywords
Short tandem repeat ; Capillary electrophoretic microdevice ; Fluorescence energy transfer ; Multiple polymerase chain reaction ; Mini Y STR haplotyping
Abstract
A fluorescence energy transfer (ET) cassette labeled mini Y short tandem repeat (STR) genotyping system is presented. A capillary electrophoretic (CE) microdevice with a cross-injector design is used to determine the fluorescence intensities of ET and single dye-labeled STR amplicons, demonstrating a 2-12 fold higher fluorescence signal of ET cassette labels than the corresponding single dye-labeled ones.Eleven extended haplotype mini Y-STRs using ET cassette labeled primers are constructed, and sensitivity and mixed sample studies are performed. Due to the improved spectroscopic properties of ET labels,multiplex mini Y-STR typing is successful with as low as 30 pg of genomic male DNA, and in the high background of female DNA. These results indicate the practical advantage of ET cassette labels for low copy number and poor-quality DNA STR genotyping to be applied for criminal investigations, paternity testing, and evolutionary studies.
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Forensic Medicine (법의학과) > 1. Journal Papers
Yonsei Authors
Shin, Kyoung Jin(신경진) ORCID logo https://orcid.org/0000-0002-1059-9665
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/107544
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