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HoxD10 gene delivery using adenovirus/adeno-associate hybrid virus inhibits the proliferation and tumorigenicity of GH4 pituitary lactotrope tumor cells.

Authors
 Mi Ae Cho  ;  Parham Yashar  ;  Suk Kyoung Kim  ;  Taewoong Noh  ;  Mary P. Gillam  ;  Eun Jig Lee  ;  J. Larry Jameson 
Citation
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.371(3) : 371-374, 2008 
Journal Title
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 
ISSN
 0006-291X 
Issue Date
2008
MeSH
Adenoviridae/genetics ; Animals ; Cell Proliferation ; Cyclin D2 ; Cyclins/metabolism ; Dependovirus/genetics ; Fibroblast Growth Factor 2/metabolism ; Gene Transfer Techniques* ; Genetic Therapy* ; Genetic Vectors* ; Homeodomain Proteins/genetics* ; Mice ; Pituitary Neoplasms/therapy* ; Prolactinoma/therapy* ; Rats ; Transcription Factors/genetics* ; beta-Galactosidase/genetics
Keywords
HoxD10 ; GH4 lactotrope tumor ; Fibroblast growth factor 2 ; Angiogenesis ; Cyclin D2 ; Adenovirus/adeno-associate virus hybrid vector
Abstract
Prolactinoma is one of the most common types of pituitary adenoma. It has been reported that a variety of growth factors and cytokines regulating cell growth and angiogenesis play an important role in the growth of prolactinoma. HoxD10 has been shown to impair endothelial cell migration, block angiogenesis, and maintain a differentiated phenotype of cells. We investigated whether HoxD10 gene delivery could inhibit the growth of prolactinoma. Rat GH4 lactotrope tumor cells were infected with adenovirus/adeno-associated virus (Ad/AAV) hybrid vectors carrying the mouse HoxD10 gene (Hyb-HoxD10) or the beta-galactosidase gene (Hyb-Gal). Hyb-HoxD10 expression inhibited GH4 cell proliferation in vitro. The expression of FGF-2 and cyclin D2 was inhibited in GH4 cells infected with Hyb-HoxD10. GH4 cells transduced with Hyb-HoxD10 did not form tumors in nude mice. These results indicate that the delivery of HoxD10 could potentially inhibit the growth of PRL-secreting tumors. This approach may be a useful tool for targeted therapy of prolactinoma and other neoplasms.
Full Text
http://www.sciencedirect.com/science/article/pii/S0006291X08007316
DOI
10.1016/j.bbrc.2008.04.085
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Lee, Eun Jig(이은직) ORCID logo https://orcid.org/0000-0002-9876-8370
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/107183
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