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Tissue-engineered blood vessels with endothelial nitric oxide synthase activity

Authors
 Sang Hyun Lim  ;  Seung-Woo Cho  ;  Jong-Chul Park  ;  Oju Jeon  ;  Jae Min Lim  ;  Sang-Soo Kim  ;  Byung-Soo Kim 
Citation
 JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, Vol.85B(2) : 537-546, 2008 
Journal Title
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
ISSN
 1552-4973 
Issue Date
2008
MeSH
Animals ; Aorta, Abdominal/enzymology ; Bioprosthesis* ; Blood Vessel Prosthesis* ; Bone Marrow Cells/metabolism* ; Cell Differentiation ; Dogs ; Endothelium, Vascular/enzymology* ; Gene Expression Regulation* ; Myocytes, Smooth Muscle/enzymology ; Nitric Oxide Synthase Type III/biosynthesis* ; Polyesters/chemistry ; Polyglycolic Acid/chemistry ; Regeneration ; Stem Cells/enzymology* ; Tissue Engineering*/methods
Keywords
vascular graft ; bone marrow-derived cell ; hybrid biodegradable polymerscaffold ; tissue engineering ; endothelial nitric oxide synthase
Abstract
Nondegradable synthetic polymer vascular grafts used in cardiovascular surgery have shown serious shortcomings, including thrombosis, calcification, infection, and lack of growth potential. Tissue engineering of vascular grafts with autologous stem cells and biodegradable polymeric materials could solve these problems. The present study is aimed to develop a tissue-engineered vascular graft (TEVG) with functional endothelium using autologous bone marrow-derived cells (BMCs) and a hybrid biodegradable polymer scaffold. Hybrid biodegradable polymer scaffolds were fabricated from poly(lactide-co-epsilon-caprolactone) (PLCL) copolymer reinforced with poly(glycolic acid) (PGA) fibers. Canine bone marrow mononuclear cells were induced in vitro to differentiate into vascular smooth muscle cells and endothelial cells. TEVGs (internal diameter: 10 mm, length: 40 mm) were fabricated by seeding vascular cells differentiated from BMCs onto PGA/PLCL scaffolds and implanted into the abdominal aorta of bone marrow donor dogs (n = 7). Eight weeks after implantation of the TEVGs, the vascular grafts remained patent. Histological and immunohistochemical analyses of the vascular grafts retrieved at 8 weeks revealed the regeneration of endothelium and smooth muscle and the presence of collagen. Western blot analysis showed that endothelial nitric oxide synthase (eNOS) was expressed in TEVGs comparable to native abdominal aortas. This study demonstrates that vascular grafts with significant eNOS activity can be tissue-engineered with autologous BMCs and hybrid biodegradable polymer scaffolds.
Full Text
http://onlinelibrary.wiley.com/doi/10.1002/jbm.b.30977/abstract
DOI
10.1002/jbm.b.30977
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/107104
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