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Paclitaxel augments cytotoxic effect of photodynamic therapy using verteporfin in gastric and bile duct cancer cells

Authors
 Seungwoo Park  ;  Sung Pil Hong  ;  Tae Yoon Oh  ;  Seungmin Bang  ;  Jae Bock Chung  ;  Si Young Song 
Citation
 PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, Vol.7(7) : 769-774, 2008 
Journal Title
 PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES 
ISSN
 1474-905X 
Issue Date
2008
MeSH
Apoptosis/drug effects ; Bile Duct Neoplasms/pathology* ; Cell Line, Tumor ; Cytochromes c/metabolism ; Dose-Response Relationship, Drug ; Humans ; Mitochondria/drug effects ; Mitochondria/metabolism ; Paclitaxel/pharmacology* ; Paclitaxel/toxicity ; Photochemotherapy* ; Photosensitizing Agents/pharmacology* ; Photosensitizing Agents/therapeutic use ; Porphyrins/pharmacology* ; Porphyrins/therapeutic use ; Stomach Neoplasms/pathology*
Keywords
Apoptosis/drug effects ; Bile Duct Neoplasms/pathology* ; Cell Line, Tumor ; Cytochromes c/metabolism ; Dose-Response Relationship, Drug ; Humans ; Mitochondria/drug effects ; Mitochondria/metabolism ; Paclitaxel/pharmacology* ; Paclitaxel/toxicity ; Photochemotherapy* ; Photosensitizing Agents/pharmacology* ; Photosensitizing Agents/therapeutic use ; Porphyrins/pharmacology* ; Porphyrins/therapeutic use ; Stomach Neoplasms/pathology*
Abstract
Photodynamic therapy (PDT) shows a limited antitumor effect in treating gastrointestinal tumors because of improper light penetration or insufficient photosensitizer uptake. The aim of this study was to evaluate the cytotoxic effect of PDT combined with paclitaxel on in vitro cancer cells. In vitro photodynamic therapy was performed in gastric cancer cells (NCI-N87) and bile duct cancer cells (YGIC-6B) using verteporfin (2 ug mL(-1)) and a PTH light source (1 000 W, Oriel Co.) with 665-675 nm narrow band pass filter. Cytotoxicity was compared using the MTT assay between cancer cells treated with PDT alone or pretreated with paclitaxel (IC(25)). Apoptotic changes were evaluated using DAPI staining, DNA fragmentation analysis, Annexin V-FITC apoptosis assay, cell cycle analysis, and western blots for cytochrome c, Bax, and Bid. The PDT-induced cytotoxicity was potentiated by pretreating with low dose paclitaxel (P < 0.001). The enhanced cytotoxicity was due to an augmented apoptotic response mediated by exaggerated cytochrome c released from mitochondria, without Bax or Bid activation. These results show that paclitaxel pretreatment enhances PDT-mediated cancer therapy.
Full Text
http://pubs.rsc.org/en/content/articlelanding/2008/pp/b719072g#!divAbstract
DOI
10.1039/b719072g
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Park, Seung Woo(박승우) ORCID logo https://orcid.org/0000-0001-8230-964X
Bang, Seungmin(방승민) ORCID logo https://orcid.org/0000-0001-5209-8351
Song, Si Young(송시영) ORCID logo https://orcid.org/0000-0002-1417-4314
Chung, Jae Bock(정재복)
Hong, Sung Pil(홍성필)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/107001
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