Cited 24 times in
Paclitaxel augments cytotoxic effect of photodynamic therapy using verteporfin in gastric and bile duct cancer cells
DC Field | Value | Language |
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dc.contributor.author | 박승우 | - |
dc.contributor.author | 방승민 | - |
dc.contributor.author | 송시영 | - |
dc.contributor.author | 정재복 | - |
dc.contributor.author | 홍성필 | - |
dc.date.accessioned | 2015-05-19T16:49:08Z | - |
dc.date.available | 2015-05-19T16:49:08Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 1474-905X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/107001 | - |
dc.description.abstract | Photodynamic therapy (PDT) shows a limited antitumor effect in treating gastrointestinal tumors because of improper light penetration or insufficient photosensitizer uptake. The aim of this study was to evaluate the cytotoxic effect of PDT combined with paclitaxel on in vitro cancer cells. In vitro photodynamic therapy was performed in gastric cancer cells (NCI-N87) and bile duct cancer cells (YGIC-6B) using verteporfin (2 ug mL(-1)) and a PTH light source (1 000 W, Oriel Co.) with 665-675 nm narrow band pass filter. Cytotoxicity was compared using the MTT assay between cancer cells treated with PDT alone or pretreated with paclitaxel (IC(25)). Apoptotic changes were evaluated using DAPI staining, DNA fragmentation analysis, Annexin V-FITC apoptosis assay, cell cycle analysis, and western blots for cytochrome c, Bax, and Bid. The PDT-induced cytotoxicity was potentiated by pretreating with low dose paclitaxel (P < 0.001). The enhanced cytotoxicity was due to an augmented apoptotic response mediated by exaggerated cytochrome c released from mitochondria, without Bax or Bid activation. These results show that paclitaxel pretreatment enhances PDT-mediated cancer therapy. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 769~774 | - |
dc.relation.isPartOf | PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Apoptosis/drug effects | - |
dc.subject.MESH | Bile Duct Neoplasms/pathology* | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Cytochromes c/metabolism | - |
dc.subject.MESH | Dose-Response Relationship, Drug | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Mitochondria/drug effects | - |
dc.subject.MESH | Mitochondria/metabolism | - |
dc.subject.MESH | Paclitaxel/pharmacology* | - |
dc.subject.MESH | Paclitaxel/toxicity | - |
dc.subject.MESH | Photochemotherapy* | - |
dc.subject.MESH | Photosensitizing Agents/pharmacology* | - |
dc.subject.MESH | Photosensitizing Agents/therapeutic use | - |
dc.subject.MESH | Porphyrins/pharmacology* | - |
dc.subject.MESH | Porphyrins/therapeutic use | - |
dc.subject.MESH | Stomach Neoplasms/pathology* | - |
dc.title | Paclitaxel augments cytotoxic effect of photodynamic therapy using verteporfin in gastric and bile duct cancer cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Seungwoo Park | - |
dc.contributor.googleauthor | Sung Pil Hong | - |
dc.contributor.googleauthor | Tae Yoon Oh | - |
dc.contributor.googleauthor | Seungmin Bang | - |
dc.contributor.googleauthor | Jae Bock Chung | - |
dc.contributor.googleauthor | Si Young Song | - |
dc.identifier.doi | 10.1039/b719072g | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01551 | - |
dc.contributor.localId | A01786 | - |
dc.contributor.localId | A02035 | - |
dc.contributor.localId | A03706 | - |
dc.contributor.localId | A04404 | - |
dc.relation.journalcode | J02516 | - |
dc.identifier.eissn | 1474-9092 | - |
dc.identifier.pmid | 18597023 | - |
dc.identifier.url | http://pubs.rsc.org/en/content/articlelanding/2008/pp/b719072g#!divAbstract | - |
dc.subject.keyword | Apoptosis/drug effects | - |
dc.subject.keyword | Bile Duct Neoplasms/pathology* | - |
dc.subject.keyword | Cell Line, Tumor | - |
dc.subject.keyword | Cytochromes c/metabolism | - |
dc.subject.keyword | Dose-Response Relationship, Drug | - |
dc.subject.keyword | Humans | - |
dc.subject.keyword | Mitochondria/drug effects | - |
dc.subject.keyword | Mitochondria/metabolism | - |
dc.subject.keyword | Paclitaxel/pharmacology* | - |
dc.subject.keyword | Paclitaxel/toxicity | - |
dc.subject.keyword | Photochemotherapy* | - |
dc.subject.keyword | Photosensitizing Agents/pharmacology* | - |
dc.subject.keyword | Photosensitizing Agents/therapeutic use | - |
dc.subject.keyword | Porphyrins/pharmacology* | - |
dc.subject.keyword | Porphyrins/therapeutic use | - |
dc.subject.keyword | Stomach Neoplasms/pathology* | - |
dc.contributor.alternativeName | Park, Seung Woo | - |
dc.contributor.alternativeName | Bang, Seung Min | - |
dc.contributor.alternativeName | Song, Si Young | - |
dc.contributor.alternativeName | Chung, Jae Bock | - |
dc.contributor.alternativeName | Hong, Sung Pil | - |
dc.contributor.affiliatedAuthor | Park, Seung Woo | - |
dc.contributor.affiliatedAuthor | Bang, Seung Min | - |
dc.contributor.affiliatedAuthor | Song, Si Young | - |
dc.contributor.affiliatedAuthor | Chung, Jae Bock | - |
dc.contributor.affiliatedAuthor | Hong, Sung Pil | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 7 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 769 | - |
dc.citation.endPage | 774 | - |
dc.identifier.bibliographicCitation | PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, Vol.7(7) : 769-774, 2008 | - |
dc.identifier.rimsid | 49456 | - |
dc.type.rims | ART | - |
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