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Vascular endothelial growth factor (VEGF) signaling regulates hippocampal neurons by elevation of intracellular calcium and activation of calcium/calmodulin protein kinase II and mammalian target of rapamycin

Authors
 Byung Woo Kim  ;  Minee Choi  ;  Yong-Seok Kim  ;  Hyungju Park  ;  Hye-Ryeon Lee  ;  Chae-Ok Yun  ;  Eun Joo Kim  ;  June-Seek Choi  ;  Sunoh Kim  ;  Hyewon Rhim  ;  Bong-Kiun Kaang  ;  Hyeon Son 
Citation
 CELLULAR SIGNALLING, Vol.20(4) : 714-725, 2008 
Journal Title
CELLULAR SIGNALLING
ISSN
 0898-6568 
Issue Date
2008
MeSH
Animals ; Astrocytes/metabolism ; Calcium Channels, L-Type/metabolism ; Calcium Signaling*/drug effects ; Calcium-Calmodulin-Dependent Protein Kinase Type 2/metabolism* ; Carrier Proteins/metabolism* ; Cells, Cultured ; Cyclic AMP Response Element-Binding Protein/metabolism ; Extracellular Signal-Regulated MAP Kinases/metabolism ; Hippocampus/drug effects ; Hippocampus/embryology ; Hippocampus/enzymology ; Hippocampus/metabolism* ; Membrane Potentials ; Nerve Tissue Proteins/biosynthesis ; Neuronal Plasticity* ; Neurons/drug effects ; Neurons/enzymology ; Neurons/metabolism* ; Phospholipase C gamma/metabolism ; Phosphotransferases (Alcohol Group Acceptor)/metabolism* ; Potassium Chloride/pharmacology ; Protein Kinases/metabolism* ; RNA, Messenger/metabolism ; Rats ; Rats, Sprague-Dawley ; Receptors, N-Methyl-D-Aspartate/metabolism ; Recombinant Proteins/metabolism ; Synaptic Transmission* ; TOR Serine-Threonine Kinases ; Time Factors ; Transcription, Genetic ; Transient Receptor Potential Channels/metabolism ; Vascular Endothelial Growth Factor A/genetics ; Vascular Endothelial Growth Factor A/metabolism* ; Vascular Endothelial Growth Factor Receptor-2/metabolism
Keywords
Rat ; Hippocampus ; VEGF ; Calcium
Abstract
The present study was undertaken to characterize neuronal activity-dependent expression and release of vascular endothelial growth factor (VEGF) from rat hippocampal neurons and its contribution to neuronal functions. Increased levels of VEGF164 mRNA were evident both in cultured neurons and slices, but not astrocytes, following membrane depolarization with KCl. Activity-dependent expression of VEGF, as well as its release, was dependent on the activation of the N-methyl-d-aspartate receptors or L-type voltage-activated calcium channels. A brief (10 min) application of recombinant VEGF165 to neurons elicited a slow rise in cytosolic Ca2+ in a VEGFR2 dependent manner. The VEGF-induced Ca2+ responses required Ca2+ influx, phospholipase Cgamma and Ca2+ stores. An inhibitor of transient receptor potential canonical channels reduced the VEGF-induced Ca2+ responses by 50%, suggesting the involvement of transient receptor potential canonical channels in the VEGF-mediated responses. The same brief stimulus with VEGF led to long-term synaptic enhancement dependent on protein synthesis. VEGF had prominent effects on the activation calcium/calmodulin protein kinase II and cAMP responsive element binding protein as well as extracellular signal-regulated protein kinase and mammalian target of rapamycin-all in a VEGFR2 dependent manner. Our findings suggest that VEGF released from neuronal cells plays a local role in Ca2+ influx and synaptic transmission that may influence the generation of long-term changes in synaptic efficacy
Full Text
http://www.sciencedirect.com/science/article/pii/S0898656807003798
DOI
10.1016/j.cellsig.2007.12.009
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
Yonsei Authors
Yun, Chae Ok(윤채옥)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/106915
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