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Androgen-dependent activation of human cytomegalovirus major immediate-early promoter in prostate cancer cells

 Jong-Seok Moon  ;  Min-Young Lee  ;  Sahng Wook Park  ;  Woong Kyu Han  ;  Soon-Won Hong  ;  Jin-Hyun Ahn  ;  Kyung-Sup Kim 
 PROSTATE, Vol.68(13) : 1450-1460, 2008 
Journal Title
Issue Date
Adenocarcinoma/genetics ; Adenocarcinoma/metabolism ; Adenocarcinoma/virology* ; Androgens/pharmacology* ; Cell Line, Tumor ; Cyclic AMP Response Element-Binding Protein ; Cyclic AMP-Dependent Protein Kinases/metabolism ; Cytomegalovirus/genetics* ; Cytomegalovirus Infections/genetics* ; Dinoprostone/metabolism ; Humans ; Male ; Metribolone/pharmacology* ; Promoter Regions, Genetic/genetics* ; Prostatic Neoplasms/genetics ; Prostatic Neoplasms/metabolism ; Prostatic Neoplasms/virology* ; Protein Kinase C/metabolism ; Protein Kinase C beta ; Receptors, Prostaglandin E/metabolism ; Transfection
prostate cancer ; androgen receptor ; human cytomegalovirus ; protein kinase A ; prostaglandin
BACKGROUND: Human cytomegalovirus (HCMV) major immediate early (MIE) promoter, which is involved in viral reactivation, is specifically activated by androgen in LNCaP prostate cancer cells. METHODS: Androgen-dependent transcriptional activities of the HCMV MIE promoter were measured by transient transfection assay. Expression levels of genes related to protein kinase A (PKA)-signaling pathway was measured by RT-PCR. RESULTS: Activation of the HCMV MIE promoter by androgen results from an increase in PKA activities. The expression level of PKA catalytic (C) subunit beta transcript variant 2 (PKA-C beta 2) was decreased by serum deprivation and was increased by R1881 treatment, in a pattern similar to changes of the HCMV MIE promoter activities. Furthermore the exogenous expression of PKA-C beta 2 strongly activated the HCMV MIE promoter, indicating that regulation of the PKA-C beta 2 expression might be a direct upstream factor in the HCMV MIE promoter regulation by androgen in LNCaP cells. CONCLUSIONS: The data elucidated the mechanisms by which androgen activates PKA in androgen-dependent prostate cancer cells, resulting in activation of the HCMV MIE promoter. These results support the possibility that activation of the HCMV MIE promoter by androgen in the prostate could cause the productive infection, which might contribute to oncomodulation in prostate cancers.
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1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Urology (비뇨의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Sup(김경섭) ORCID logo https://orcid.org/0000-0001-8483-8537
Moon, Jong-Seok(문종석)
Park, Sahng Wook(박상욱) ORCID logo https://orcid.org/0000-0002-9594-7074
Lee, Min Young(이민영)
Han, Woong Kyu(한웅규) ORCID logo https://orcid.org/0000-0002-2527-4046
Hong, Soon Won(홍순원) ORCID logo https://orcid.org/0000-0002-0324-2414
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