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Androgen-dependent activation of human cytomegalovirus major immediate-early promoter in prostate cancer cells

DC FieldValueLanguage
dc.contributor.author한웅규-
dc.contributor.author홍순원-
dc.contributor.author김경섭-
dc.contributor.author문종석-
dc.contributor.author박상욱-
dc.contributor.author이민영-
dc.date.accessioned2015-05-19T16:31:30Z-
dc.date.available2015-05-19T16:31:30Z-
dc.date.issued2008-
dc.identifier.issn0270-4137-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/106466-
dc.description.abstractBACKGROUND: Human cytomegalovirus (HCMV) major immediate early (MIE) promoter, which is involved in viral reactivation, is specifically activated by androgen in LNCaP prostate cancer cells. METHODS: Androgen-dependent transcriptional activities of the HCMV MIE promoter were measured by transient transfection assay. Expression levels of genes related to protein kinase A (PKA)-signaling pathway was measured by RT-PCR. RESULTS: Activation of the HCMV MIE promoter by androgen results from an increase in PKA activities. The expression level of PKA catalytic (C) subunit beta transcript variant 2 (PKA-C beta 2) was decreased by serum deprivation and was increased by R1881 treatment, in a pattern similar to changes of the HCMV MIE promoter activities. Furthermore the exogenous expression of PKA-C beta 2 strongly activated the HCMV MIE promoter, indicating that regulation of the PKA-C beta 2 expression might be a direct upstream factor in the HCMV MIE promoter regulation by androgen in LNCaP cells. CONCLUSIONS: The data elucidated the mechanisms by which androgen activates PKA in androgen-dependent prostate cancer cells, resulting in activation of the HCMV MIE promoter. These results support the possibility that activation of the HCMV MIE promoter by androgen in the prostate could cause the productive infection, which might contribute to oncomodulation in prostate cancers.-
dc.description.statementOfResponsibilityopen-
dc.format.extent1450~1460-
dc.relation.isPartOfPROSTATE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdenocarcinoma/genetics-
dc.subject.MESHAdenocarcinoma/metabolism-
dc.subject.MESHAdenocarcinoma/virology*-
dc.subject.MESHAndrogens/pharmacology*-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHCyclic AMP Response Element-Binding Protein-
dc.subject.MESHCyclic AMP-Dependent Protein Kinases/metabolism-
dc.subject.MESHCytomegalovirus/genetics*-
dc.subject.MESHCytomegalovirus Infections/genetics*-
dc.subject.MESHDinoprostone/metabolism-
dc.subject.MESHHumans-
dc.subject.MESHMale-
dc.subject.MESHMetribolone/pharmacology*-
dc.subject.MESHPromoter Regions, Genetic/genetics*-
dc.subject.MESHProstatic Neoplasms/genetics-
dc.subject.MESHProstatic Neoplasms/metabolism-
dc.subject.MESHProstatic Neoplasms/virology*-
dc.subject.MESHProtein Kinase C/metabolism-
dc.subject.MESHProtein Kinase C beta-
dc.subject.MESHReceptors, Prostaglandin E/metabolism-
dc.subject.MESHTransfection-
dc.titleAndrogen-dependent activation of human cytomegalovirus major immediate-early promoter in prostate cancer cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Urology (비뇨기과학)-
dc.contributor.googleauthorJong-Seok Moon-
dc.contributor.googleauthorMin-Young Lee-
dc.contributor.googleauthorSahng Wook Park-
dc.contributor.googleauthorWoong Kyu Han-
dc.contributor.googleauthorSoon-Won Hong-
dc.contributor.googleauthorJin-Hyun Ahn-
dc.contributor.googleauthorKyung-Sup Kim-
dc.identifier.doi10.1002/pros.20817-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA04308-
dc.contributor.localIdA04411-
dc.contributor.localIdA00297-
dc.contributor.localIdA01487-
dc.contributor.localIdA02783-
dc.contributor.localIdA01381-1-
dc.relation.journalcodeJ02557-
dc.identifier.eissn1097-0045-
dc.identifier.pmid18615458-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1002/pros.20817/abstract-
dc.subject.keywordprostate cancer-
dc.subject.keywordandrogen receptor-
dc.subject.keywordhuman cytomegalovirus-
dc.subject.keywordprotein kinase A-
dc.subject.keywordprostaglandin-
dc.contributor.alternativeNameHan, Woong Kyu-
dc.contributor.alternativeNameHong, Soon Won-
dc.contributor.alternativeNameKim, Kyung Sup-
dc.contributor.alternativeNameMoon, Jong-Seok-
dc.contributor.alternativeNamePark, Sahng Wook-
dc.contributor.alternativeNameLee, Min Young-
dc.contributor.affiliatedAuthorHan, Woong Kyu-
dc.contributor.affiliatedAuthorHong, Soon Won-
dc.contributor.affiliatedAuthorKim, Kyung Sup-
dc.contributor.affiliatedAuthorPark, Sahng Wook-
dc.contributor.affiliatedAuthorLee, Min Young-
dc.contributor.affiliatedAuthorMoon, Jong-Seok-
dc.rights.accessRightsnot free-
dc.citation.volume68-
dc.citation.number13-
dc.citation.startPage1450-
dc.citation.endPage1460-
dc.identifier.bibliographicCitationPROSTATE, Vol.68(13) : 1450-1460, 2008-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Urology (비뇨의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers

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