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Expression of neurotrophic factors in human primary pterygeal tissue and selective TNF-alpha-induced stimulation of ciliary neurotrophic factor in pterygeal fibroblasts

Authors
 Samin Hong  ;  Jin Young Choi  ;  Hyung Keun Lee  ;  Gong Je Seong  ;  Kyoung Yul Seo  ;  Eung-Kweon Kim  ;  Suk Ho Byeon 
Citation
 EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY , Vol.60(6) : 513-520, 2008 
Journal Title
 EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY 
ISSN
 0940-2993 
Issue Date
2008
MeSH
Brain-Derived Neurotrophic Factor/genetics ; Brain-Derived Neurotrophic Factor/metabolism ; Cells, Cultured ; Ciliary Neurotrophic Factor/genetics ; Ciliary Neurotrophic Factor/metabolism ; Fibroblasts/drug effects ; Fibroblasts/metabolism* ; Fibroblasts/pathology ; Gene Expression/drug effects ; Humans ; Limbus Corneae/cytology ; Limbus Corneae/drug effects ; Limbus Corneae/metabolism ; Nerve Growth Factor/genetics ; Nerve Growth Factor/metabolism ; Nerve Growth Factors/genetics ; Nerve Growth Factors/metabolism* ; Pterygium/drug therapy ; Pterygium/metabolism* ; Pterygium/pathology ; RNA, Messenger/metabolism ; Recombinant Proteins/pharmacology ; Tumor Necrosis Factor-alpha/pharmacology*
Keywords
Pterygium ; Neurotrophic factor ; Ciliary neurotrophic factor ; Tumor-necrosis factor
Abstract
Pterygium is a degenerative, fibrovascular and hyperplastic growth resulting from a proliferative tissue actively growing from altered limbal stem cells at the corneal-conjunctival junction. However, the pathogenesis of pterygium is still unclear. The aim of this study was to determine the expression of neurotrophic factors (nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), ciliary neurotrophic factor (CNTF), and neurotrophin (NT)-3, NT-4/5) in pterygeal tissue and to confirm whether tumor-necrosis factor-alpha (TNF-alpha) modulates the expression of these neurotrophic factors in primary cultured pterygeal fibroblasts. We looked for expression of these neurotrophic factors in surgically excised pterygia and normal donor limbal tissues by immunohistochemistry and in primary cultured pterygeal and normal subconjunctival Tenon's fibroblasts by Western blot and reverse transcription-polymerase chain reaction (RT-PCR). Expression of CNTF and NT-4/5 is increased in pterygeal tissues compared with normal tissue, and production of CNTF is stimulated in pterygeal fibroblasts by TNF-alpha but not in normal fibroblasts. This selective stimulation of CNTF in pterygeal fibroblasts suggests that CNTF may play a role in the pathogenesis of pterygium
Full Text
http://www.sciencedirect.com/science/article/pii/S0940299308000857
DOI
10.1016/j.etp.2008.04.013
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Eung Kweon(김응권) ORCID logo https://orcid.org/0000-0002-1453-8042
Byeon, Suk Ho(변석호) ORCID logo https://orcid.org/0000-0001-8101-0830
Seo, Kyuong Yul(서경률) ORCID logo https://orcid.org/0000-0002-9855-1980
Seong, Gong Je(성공제) ORCID logo https://orcid.org/0000-0002-5456-4296
Lee, Hyung Keun(이형근) ORCID logo https://orcid.org/0000-0002-1123-2136
Hong, Sa Min(홍사민)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/106455
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