A modified mini-primer set for analyzing mitochondrial DNA control region sequences from highly degraded forensic samples

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Authors: Hwan Young Lee ; Na Young Kim ; Myung Jin Park ; Woo Ick Yang ; Kyoung-Jin Shin

MeSH: DNA Primers* ; DNA, Mitochondrial/analysis* ; DNA, Mitochondrial/chemistry ; Forensic Anthropology/methods* ; Humans ; Nucleic Acid Amplification Techniques ; Polymerase Chain Reaction ; Sequence Analysis, DNA* ; Time Factors

Keywords: DNA Primers* ; DNA, Mitochondrial/analysis* ; DNA, Mitochondrial/chemistry ; Forensic Anthropology/methods* ; Humans ; Nucleic Acid Amplification Techniques ; Polymerase Chain Reaction ; Sequence Analysis, DNA* ; Time Factors

Abstract: To facilitate the analysis of mitochondrial DNA (mtDNA) control region sequences from highly degraded skeletal remains, a modified mini-primer set was designed to overcome the limitations of the Armed Forces DNA Identification Laboratory (AFDIL) mini-primer set. This modified mini-primer set is less affected by nucleotide variability and PCR amplification conditions than the AFDIL mini-primer set, and was able to amplify the mtDNA sequences of 55-year-old skeletal remains with high efficiency, indicating that it is a useful tool for analyzing mtDNA control region sequences from highly degraded forensic samples.