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A modified mini-primer set for analyzing mitochondrial DNA control region sequences from highly degraded forensic samples

Authors
 Hwan Young Lee  ;  Na Young Kim  ;  Myung Jin Park  ;  Woo Ick Yang  ;  Kyoung-Jin Shin 
Citation
 BIOTECHNIQUES, Vol.44(4) : 555-558, 2008 
Journal Title
BIOTECHNIQUES
ISSN
 0736-6205 
Issue Date
2008
MeSH
DNA Primers* ; DNA, Mitochondrial/analysis* ; DNA, Mitochondrial/chemistry ; Forensic Anthropology/methods* ; Humans ; Nucleic Acid Amplification Techniques ; Polymerase Chain Reaction ; Sequence Analysis, DNA* ; Time Factors
Keywords
DNA Primers* ; DNA, Mitochondrial/analysis* ; DNA, Mitochondrial/chemistry ; Forensic Anthropology/methods* ; Humans ; Nucleic Acid Amplification Techniques ; Polymerase Chain Reaction ; Sequence Analysis, DNA* ; Time Factors
Abstract
To facilitate the analysis of mitochondrial DNA (mtDNA) control region sequences from highly degraded skeletal remains, a modified mini-primer set was designed to overcome the limitations of the Armed Forces DNA Identification Laboratory (AFDIL) mini-primer set. This modified mini-primer set is less affected by nucleotide variability and PCR amplification conditions than the AFDIL mini-primer set, and was able to amplify the mtDNA sequences of 55-year-old skeletal remains with high efficiency, indicating that it is a useful tool for analyzing mtDNA control region sequences from highly degraded forensic samples.
Files in This Item:
T200800303.pdf Download
DOI
10.2144/000112672
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Forensic Medicine (법의학과) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Na Young(김나영)
Park, Myung Jin(박명진)
Shin, Kyoung Jin(신경진) ORCID logo https://orcid.org/0000-0002-1059-9665
Yang, Woo Ick(양우익) ORCID logo https://orcid.org/0000-0002-6084-5019
Lee, Hwan Young(이환영)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/106449
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