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Effects of mycophenolic acid on high glucose-induced fibronectin secretion and cellular reactive oxygen species in rat vascular smooth muscle cells

Authors
 J. Park  ;  M.K. Ju  ;  J.S. Song  ;  K.H. Huh  ;  M.S. Kim  ;  Y.S. Kim 
Citation
 TRANSPLANTATION PROCEEDINGS, Vol.40(2) : 467-469, 2008 
Journal Title
 TRANSPLANTATION PROCEEDINGS 
ISSN
 0041-1345 
Issue Date
2008
MeSH
Animals ; Cell Culture Techniques ; Cell Division ; Fibronectins/secretion* ; Flow Cytometry ; Glucose/pharmacology* ; Muscle, Smooth, Vascular/cytology ; Muscle, Smooth, Vascular/drug effects ; Muscle, Smooth, Vascular/physiology* ; Mycophenolic Acid/pharmacology* ; Rats ; Reactive Oxygen Species/metabolism*
Keywords
Animals ; Cell Culture Techniques ; Cell Division ; Fibronectins/secretion* ; Flow Cytometry ; Glucose/pharmacology* ; Muscle, Smooth, Vascular/cytology ; Muscle, Smooth, Vascular/drug effects ; Muscle, Smooth, Vascular/physiology* ; Mycophenolic Acid/pharmacology* ; Rats ; Reactive Oxygen Species/metabolism*
Abstract
Vascular smooth muscle cell (VSMC) proliferation, migration, and matrix protein accumulation play important roles in the development and progression of vascular disease including diabetic vascular complications and chronic allograft vasculopathy. Mycophenolic acid (MPA) inhibits various mesenchymal cell proliferation and matrix protein accumulation and reactive oxygen species (ROS). In this study, we investigated the effects of MPA on high glucose (HG)-induced fibronectin secretion and the role of ROS in rat VSMCs. Primary cultured rat VSMCs from Sprague-Dawley rats were exposed for 1 hour before stimulation with media containing 5.6 mmol/L glucose (low glucose [LG]), 30 mmol/L mannitol (M), or 30 mmol/L glucose (HG) with or without MPA (0.1-10 micromol/L) or N-acetylcysteine (NAC; 5 mmol/L). Fibronectin secretion was measured by Western blot analysis and dichlorofluorescein (DCF)-sensitive cellular ROS by flow cytometry. HG significantly increased fibronectin secretion by 1.7-fold. The increment of DCF-sensitive cellular ROS was 1.5-fold at 1 hour by HG. MPA at concentrations above 1 micromol/L effectively inhibited HG-induced fibronectin secretion and cellular ROS in a dose-dependent manner. NAC at 5 mmol/L also inhibited HG-induced rat VSMC activation. These results suggested that MPA inhibits HG-induced VSMC activation partially through inhibiting cellular ROS
Full Text
http://www.sciencedirect.com/science/article/pii/S004113450800016X
DOI
10.1016/j.transproceed.2008.01.015
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Myoung Soo(김명수) ORCID logo https://orcid.org/0000-0002-8975-8381
Kim, Yu Seun(김유선) ORCID logo https://orcid.org/0000-0002-5105-1567
Joo, Man Ki(주만기) ORCID logo https://orcid.org/0000-0002-4112-7003
Huh, Kyu Ha(허규하) ORCID logo https://orcid.org/0000-0003-1364-6989
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/106285
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