Cited 1 times in
Effects of mycophenolic acid on high glucose-induced fibronectin secretion and cellular reactive oxygen species in rat vascular smooth muscle cells
DC Field | Value | Language |
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dc.contributor.author | 김명수 | - |
dc.contributor.author | 김유선 | - |
dc.contributor.author | 주만기 | - |
dc.contributor.author | 허규하 | - |
dc.date.accessioned | 2015-05-19T16:25:40Z | - |
dc.date.available | 2015-05-19T16:25:40Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 0041-1345 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/106285 | - |
dc.description.abstract | Vascular smooth muscle cell (VSMC) proliferation, migration, and matrix protein accumulation play important roles in the development and progression of vascular disease including diabetic vascular complications and chronic allograft vasculopathy. Mycophenolic acid (MPA) inhibits various mesenchymal cell proliferation and matrix protein accumulation and reactive oxygen species (ROS). In this study, we investigated the effects of MPA on high glucose (HG)-induced fibronectin secretion and the role of ROS in rat VSMCs. Primary cultured rat VSMCs from Sprague-Dawley rats were exposed for 1 hour before stimulation with media containing 5.6 mmol/L glucose (low glucose [LG]), 30 mmol/L mannitol (M), or 30 mmol/L glucose (HG) with or without MPA (0.1-10 micromol/L) or N-acetylcysteine (NAC; 5 mmol/L). Fibronectin secretion was measured by Western blot analysis and dichlorofluorescein (DCF)-sensitive cellular ROS by flow cytometry. HG significantly increased fibronectin secretion by 1.7-fold. The increment of DCF-sensitive cellular ROS was 1.5-fold at 1 hour by HG. MPA at concentrations above 1 micromol/L effectively inhibited HG-induced fibronectin secretion and cellular ROS in a dose-dependent manner. NAC at 5 mmol/L also inhibited HG-induced rat VSMC activation. These results suggested that MPA inhibits HG-induced VSMC activation partially through inhibiting cellular ROS | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 467~469 | - |
dc.relation.isPartOf | TRANSPLANTATION PROCEEDINGS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Culture Techniques | - |
dc.subject.MESH | Cell Division | - |
dc.subject.MESH | Fibronectins/secretion* | - |
dc.subject.MESH | Flow Cytometry | - |
dc.subject.MESH | Glucose/pharmacology* | - |
dc.subject.MESH | Muscle, Smooth, Vascular/cytology | - |
dc.subject.MESH | Muscle, Smooth, Vascular/drug effects | - |
dc.subject.MESH | Muscle, Smooth, Vascular/physiology* | - |
dc.subject.MESH | Mycophenolic Acid/pharmacology* | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Reactive Oxygen Species/metabolism* | - |
dc.title | Effects of mycophenolic acid on high glucose-induced fibronectin secretion and cellular reactive oxygen species in rat vascular smooth muscle cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Surgery (외과학) | - |
dc.contributor.googleauthor | J. Park | - |
dc.contributor.googleauthor | M.K. Ju | - |
dc.contributor.googleauthor | J.S. Song | - |
dc.contributor.googleauthor | K.H. Huh | - |
dc.contributor.googleauthor | M.S. Kim | - |
dc.contributor.googleauthor | Y.S. Kim | - |
dc.identifier.doi | 10.1016/j.transproceed.2008.01.015 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00424 | - |
dc.contributor.localId | A00785 | - |
dc.contributor.localId | A03949 | - |
dc.contributor.localId | A04344 | - |
dc.relation.journalcode | J02755 | - |
dc.identifier.eissn | 1873-2623 | - |
dc.identifier.pmid | 18374104 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S004113450800016X | - |
dc.subject.keyword | Animals | - |
dc.subject.keyword | Cell Culture Techniques | - |
dc.subject.keyword | Cell Division | - |
dc.subject.keyword | Fibronectins/secretion* | - |
dc.subject.keyword | Flow Cytometry | - |
dc.subject.keyword | Glucose/pharmacology* | - |
dc.subject.keyword | Muscle, Smooth, Vascular/cytology | - |
dc.subject.keyword | Muscle, Smooth, Vascular/drug effects | - |
dc.subject.keyword | Muscle, Smooth, Vascular/physiology* | - |
dc.subject.keyword | Mycophenolic Acid/pharmacology* | - |
dc.subject.keyword | Rats | - |
dc.subject.keyword | Reactive Oxygen Species/metabolism* | - |
dc.contributor.alternativeName | Kim, Myoung Soo | - |
dc.contributor.alternativeName | Kim, Yu Seun | - |
dc.contributor.alternativeName | Joo, Man Ki | - |
dc.contributor.alternativeName | Huh, Kyu Ha | - |
dc.contributor.affiliatedAuthor | Kim, Myoung Soo | - |
dc.contributor.affiliatedAuthor | Kim, Yu Seun | - |
dc.contributor.affiliatedAuthor | Joo, Man Ki | - |
dc.contributor.affiliatedAuthor | Huh, Kyu Ha | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 40 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 467 | - |
dc.citation.endPage | 469 | - |
dc.identifier.bibliographicCitation | TRANSPLANTATION PROCEEDINGS, Vol.40(2) : 467-469, 2008 | - |
dc.identifier.rimsid | 56327 | - |
dc.type.rims | ART | - |
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