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Effects of mycophenolic acid on high glucose-induced fibronectin secretion and cellular reactive oxygen species in rat vascular smooth muscle cells

DC Field Value Language
dc.contributor.author김명수-
dc.contributor.author김유선-
dc.contributor.author주만기-
dc.contributor.author허규하-
dc.date.accessioned2015-05-19T16:25:40Z-
dc.date.available2015-05-19T16:25:40Z-
dc.date.issued2008-
dc.identifier.issn0041-1345-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/106285-
dc.description.abstractVascular smooth muscle cell (VSMC) proliferation, migration, and matrix protein accumulation play important roles in the development and progression of vascular disease including diabetic vascular complications and chronic allograft vasculopathy. Mycophenolic acid (MPA) inhibits various mesenchymal cell proliferation and matrix protein accumulation and reactive oxygen species (ROS). In this study, we investigated the effects of MPA on high glucose (HG)-induced fibronectin secretion and the role of ROS in rat VSMCs. Primary cultured rat VSMCs from Sprague-Dawley rats were exposed for 1 hour before stimulation with media containing 5.6 mmol/L glucose (low glucose [LG]), 30 mmol/L mannitol (M), or 30 mmol/L glucose (HG) with or without MPA (0.1-10 micromol/L) or N-acetylcysteine (NAC; 5 mmol/L). Fibronectin secretion was measured by Western blot analysis and dichlorofluorescein (DCF)-sensitive cellular ROS by flow cytometry. HG significantly increased fibronectin secretion by 1.7-fold. The increment of DCF-sensitive cellular ROS was 1.5-fold at 1 hour by HG. MPA at concentrations above 1 micromol/L effectively inhibited HG-induced fibronectin secretion and cellular ROS in a dose-dependent manner. NAC at 5 mmol/L also inhibited HG-induced rat VSMC activation. These results suggested that MPA inhibits HG-induced VSMC activation partially through inhibiting cellular ROS-
dc.description.statementOfResponsibilityopen-
dc.format.extent467~469-
dc.relation.isPartOfTRANSPLANTATION PROCEEDINGS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHCell Culture Techniques-
dc.subject.MESHCell Division-
dc.subject.MESHFibronectins/secretion*-
dc.subject.MESHFlow Cytometry-
dc.subject.MESHGlucose/pharmacology*-
dc.subject.MESHMuscle, Smooth, Vascular/cytology-
dc.subject.MESHMuscle, Smooth, Vascular/drug effects-
dc.subject.MESHMuscle, Smooth, Vascular/physiology*-
dc.subject.MESHMycophenolic Acid/pharmacology*-
dc.subject.MESHRats-
dc.subject.MESHReactive Oxygen Species/metabolism*-
dc.titleEffects of mycophenolic acid on high glucose-induced fibronectin secretion and cellular reactive oxygen species in rat vascular smooth muscle cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Surgery (외과학)-
dc.contributor.googleauthorJ. Park-
dc.contributor.googleauthorM.K. Ju-
dc.contributor.googleauthorJ.S. Song-
dc.contributor.googleauthorK.H. Huh-
dc.contributor.googleauthorM.S. Kim-
dc.contributor.googleauthorY.S. Kim-
dc.identifier.doi10.1016/j.transproceed.2008.01.015-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00424-
dc.contributor.localIdA00785-
dc.contributor.localIdA03949-
dc.contributor.localIdA04344-
dc.relation.journalcodeJ02755-
dc.identifier.eissn1873-2623-
dc.identifier.pmid18374104-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S004113450800016X-
dc.subject.keywordAnimals-
dc.subject.keywordCell Culture Techniques-
dc.subject.keywordCell Division-
dc.subject.keywordFibronectins/secretion*-
dc.subject.keywordFlow Cytometry-
dc.subject.keywordGlucose/pharmacology*-
dc.subject.keywordMuscle, Smooth, Vascular/cytology-
dc.subject.keywordMuscle, Smooth, Vascular/drug effects-
dc.subject.keywordMuscle, Smooth, Vascular/physiology*-
dc.subject.keywordMycophenolic Acid/pharmacology*-
dc.subject.keywordRats-
dc.subject.keywordReactive Oxygen Species/metabolism*-
dc.contributor.alternativeNameKim, Myoung Soo-
dc.contributor.alternativeNameKim, Yu Seun-
dc.contributor.alternativeNameJoo, Man Ki-
dc.contributor.alternativeNameHuh, Kyu Ha-
dc.contributor.affiliatedAuthorKim, Myoung Soo-
dc.contributor.affiliatedAuthorKim, Yu Seun-
dc.contributor.affiliatedAuthorJoo, Man Ki-
dc.contributor.affiliatedAuthorHuh, Kyu Ha-
dc.rights.accessRightsnot free-
dc.citation.volume40-
dc.citation.number2-
dc.citation.startPage467-
dc.citation.endPage469-
dc.identifier.bibliographicCitationTRANSPLANTATION PROCEEDINGS, Vol.40(2) : 467-469, 2008-
dc.identifier.rimsid56327-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers

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