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Mitochondrial ATP synthase is a target for TNBS-induced protein carbonylation in XS-106 dendritic cells

 Jeong Hwan Je  ;  Tae Hyung Lee  ;  Dong Hyun Kim  ;  Young Hun Cho  ;  Ju Hee Lee  ;  Soo Chan Kim  ;  Sang-Kyou Lee  ;  Jaewon Lee  ;  Min-Geol Lee 
 PROTEOMICS, Vol.8(12) : 2384-2393, 2008 
Journal Title
Issue Date
Animals ; Animals, Newborn ; Cell Culture Techniques ; Cell Line ; Culture Media, Conditioned ; Dendritic Cells/drug effects* ; Dendritic Cells/metabolism ; Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology ; Humans ; Mice ; Mitochondrial Proton-Translocating ATPases/metabolism* ; Monocytes/cytology ; Peroxidase/genetics ; Peroxidase/metabolism ; Protein Carbonylation/drug effects* ; Proteomics/methods ; Reactive Oxygen Species/metabolism ; Skin/cytology ; Trinitrobenzenesulfonic Acid/pharmacology*
ATP synthase ; Protein carbonylation ; ROS ; TNBS ; XS‐106
ROS are produced in dendritic cells (DCs) during antigen presentation in contact hypersensitivity (CHS). As a result, ROS cause a number of nonenzymatic protein modifications, including carbonylation, which is the most widely used marker of oxidative stress. 2,4,6-Trinitrobenzene sulfonic acid (TNBS) is a well-characterized contact allergen that results in the formation of ROS. However, proteins that are carbonylated in DCs in response to TNBS have not been identified. To study ROS-dependent protein carbonylation in response to TNBS, we used the well-established mouse DC line, XS-106. We focused on the effects of TNBS on oxidation by examining selected oxidative markers. We identified TNBS-induced ROS and myeloperoxidase (MPO) proteins and demonstrated that the increase in ROS resulted in IL-12 production. The increase in oxidation was further confirmed by an oxidation-dependent increase in protein modifications, such as carbonylation. In fact, TNBS strongly induced carbonylation of mitochondrial adenosine triphosphate (ATP) synthase in XS-106 DCs, as determined by MALDI-TOF analysis and 2-D Western blotting. ROS production and protein carbonylation were confirmed in human monocyte-derived DCs (Mo-DCs). Furthermore, glutathione (GSH) decreased ROS and protein carbonylation in Mo-DCs. Carbonylation of ATP synthase in DCs may contribute to the pathophysiology of CHS.
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1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Dong Hyun(김동현)
Kim, Soo Chan(김수찬) ORCID logo https://orcid.org/0000-0002-2327-4755
Lee, Min Geol(이민걸) ORCID logo https://orcid.org/0000-0001-7040-5335
Lee, Ju Hee(이주희) ORCID logo https://orcid.org/0000-0002-1739-5956
Cho, Young Hun(조영훈)
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