Cited 22 times in
Mitochondrial ATP synthase is a target for TNBS-induced protein carbonylation in XS-106 dendritic cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김동현 | - |
dc.contributor.author | 김수찬 | - |
dc.contributor.author | 이민걸 | - |
dc.contributor.author | 이주희 | - |
dc.contributor.author | 조영훈 | - |
dc.date.accessioned | 2015-05-19T16:24:37Z | - |
dc.date.available | 2015-05-19T16:24:37Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 1615-9853 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/106254 | - |
dc.description.abstract | ROS are produced in dendritic cells (DCs) during antigen presentation in contact hypersensitivity (CHS). As a result, ROS cause a number of nonenzymatic protein modifications, including carbonylation, which is the most widely used marker of oxidative stress. 2,4,6-Trinitrobenzene sulfonic acid (TNBS) is a well-characterized contact allergen that results in the formation of ROS. However, proteins that are carbonylated in DCs in response to TNBS have not been identified. To study ROS-dependent protein carbonylation in response to TNBS, we used the well-established mouse DC line, XS-106. We focused on the effects of TNBS on oxidation by examining selected oxidative markers. We identified TNBS-induced ROS and myeloperoxidase (MPO) proteins and demonstrated that the increase in ROS resulted in IL-12 production. The increase in oxidation was further confirmed by an oxidation-dependent increase in protein modifications, such as carbonylation. In fact, TNBS strongly induced carbonylation of mitochondrial adenosine triphosphate (ATP) synthase in XS-106 DCs, as determined by MALDI-TOF analysis and 2-D Western blotting. ROS production and protein carbonylation were confirmed in human monocyte-derived DCs (Mo-DCs). Furthermore, glutathione (GSH) decreased ROS and protein carbonylation in Mo-DCs. Carbonylation of ATP synthase in DCs may contribute to the pathophysiology of CHS. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 2384~2393 | - |
dc.relation.isPartOf | PROTEOMICS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Animals, Newborn | - |
dc.subject.MESH | Cell Culture Techniques | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Culture Media, Conditioned | - |
dc.subject.MESH | Dendritic Cells/drug effects* | - |
dc.subject.MESH | Dendritic Cells/metabolism | - |
dc.subject.MESH | Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mitochondrial Proton-Translocating ATPases/metabolism* | - |
dc.subject.MESH | Monocytes/cytology | - |
dc.subject.MESH | Peroxidase/genetics | - |
dc.subject.MESH | Peroxidase/metabolism | - |
dc.subject.MESH | Protein Carbonylation/drug effects* | - |
dc.subject.MESH | Proteomics/methods | - |
dc.subject.MESH | Reactive Oxygen Species/metabolism | - |
dc.subject.MESH | Skin/cytology | - |
dc.subject.MESH | Trinitrobenzenesulfonic Acid/pharmacology* | - |
dc.title | Mitochondrial ATP synthase is a target for TNBS-induced protein carbonylation in XS-106 dendritic cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Dermatology (피부과학) | - |
dc.contributor.googleauthor | Jeong Hwan Je | - |
dc.contributor.googleauthor | Tae Hyung Lee | - |
dc.contributor.googleauthor | Dong Hyun Kim | - |
dc.contributor.googleauthor | Young Hun Cho | - |
dc.contributor.googleauthor | Ju Hee Lee | - |
dc.contributor.googleauthor | Soo Chan Kim | - |
dc.contributor.googleauthor | Sang-Kyou Lee | - |
dc.contributor.googleauthor | Jaewon Lee | - |
dc.contributor.googleauthor | Min-Geol Lee | - |
dc.identifier.doi | 10.1002/pmic.200700962 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02779 | - |
dc.contributor.localId | A03171 | - |
dc.contributor.localId | A03861 | - |
dc.contributor.localId | A00637 | - |
dc.contributor.localId | A00412 | - |
dc.relation.journalcode | J02566 | - |
dc.identifier.eissn | 1615-9861 | - |
dc.identifier.pmid | 18563732 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1002/pmic.200700962/abstract | - |
dc.subject.keyword | ATP synthase | - |
dc.subject.keyword | Protein carbonylation | - |
dc.subject.keyword | ROS | - |
dc.subject.keyword | TNBS | - |
dc.subject.keyword | XS‐106 | - |
dc.contributor.alternativeName | Kim, Dong Hyun | - |
dc.contributor.alternativeName | Kim, Soo Chan | - |
dc.contributor.alternativeName | Lee, Min Geol | - |
dc.contributor.alternativeName | Lee, Ju Hee | - |
dc.contributor.alternativeName | Cho, Young Hun | - |
dc.contributor.affiliatedAuthor | Lee, Min Geol | - |
dc.contributor.affiliatedAuthor | Lee, Ju Hee | - |
dc.contributor.affiliatedAuthor | Cho, Young Hun | - |
dc.contributor.affiliatedAuthor | Kim, Soo Chan | - |
dc.contributor.affiliatedAuthor | Kim, Dong Hyun | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 8 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 2384 | - |
dc.citation.endPage | 2393 | - |
dc.identifier.bibliographicCitation | PROTEOMICS, Vol.8(12) : 2384-2393, 2008 | - |
dc.identifier.rimsid | 56312 | - |
dc.type.rims | ART | - |
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