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Physical interactions and functional coupling between Daxx and sodium hydrogen exchanger 1 in ischemic cell death
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김은희 | - |
dc.contributor.author | 김준오 | - |
dc.contributor.author | 김혜영 | - |
dc.contributor.author | 이민구 | - |
dc.date.accessioned | 2015-05-19T16:20:35Z | - |
dc.date.available | 2015-05-19T16:20:35Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/106132 | - |
dc.description.abstract | Daxx, a death domain-associated protein, is implicated in ischemic cell death. To clarify the mechanism of cell death mediated by Daxx, a yeast two-hybrid assay was performed. Sodium hydrogen exchanger isoform 1 (NHE1) was identified as a Daxx-interacting protein. During ischemic stress, Daxx translocates from the nucleus to the cytoplasm, where it colocalizes with NHE1. Daxx binds to the ezrin/radixin/moesin-interacting domain of NHE1, in competition with ezrin. Consistent with this finding, transfection of the constitutively cytoplasmic mutant, Daxx(W621A), inhibited ezrin-mediated Akt-1 activation. Moreover, transfection of Daxx(W621A), but not the Daxx(S667A) mutant that is confined to the nucleus, accelerated pH(i) recovery from an acid load, indicating that the cytoplasmic protein activates NHE1. Based on the results, we propose that ischemic insult triggers the nucleocytoplasmic translocation of Daxx, following which cytoplasmic Daxx stimulates the NHE1 transporter activity and suppresses activation of the NHE1-ezrin-Akt-1 pathway. Our data support a novel molecular function of Daxx as an upstream regulator of NHE1 in ischemic cell death | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1018~1025 | - |
dc.relation.isPartOf | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adult | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Lens Implantation, Intraocular/methods* | - |
dc.subject.MESH | Lenses, Intraocular* | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Marfan Syndrome/complications | - |
dc.subject.MESH | Pupil Disorders/etiology* | - |
dc.subject.MESH | Recurrence | - |
dc.subject.MESH | Sclera/surgery* | - |
dc.subject.MESH | Silicone Elastomers* | - |
dc.subject.MESH | Suture Techniques | - |
dc.subject.MESH | Visual Acuity | - |
dc.title | Physical interactions and functional coupling between Daxx and sodium hydrogen exchanger 1 in ischemic cell death | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Medical Research Center (임상의학연구센터) | - |
dc.contributor.googleauthor | Yong-SamJung | - |
dc.contributor.googleauthor | Hye-Young Kim | - |
dc.contributor.googleauthor | Juno Kim | - |
dc.contributor.googleauthor | Min-Goo Lee | - |
dc.contributor.googleauthor | Jacques Pouysse´gur | - |
dc.contributor.googleauthor | Eunhee Kim | - |
dc.identifier.doi | 10.1074/jbc.M707722200 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00830 | - |
dc.contributor.localId | A00957 | - |
dc.contributor.localId | A01175 | - |
dc.contributor.localId | A02781 | - |
dc.relation.journalcode | J01258 | - |
dc.identifier.eissn | 1083-351X | - |
dc.identifier.pmid | 18003619 | - |
dc.subject.keyword | Adult | - |
dc.subject.keyword | Humans | - |
dc.subject.keyword | Lens Implantation, Intraocular/methods* | - |
dc.subject.keyword | Lenses, Intraocular* | - |
dc.subject.keyword | Male | - |
dc.subject.keyword | Marfan Syndrome/complications | - |
dc.subject.keyword | Pupil Disorders/etiology* | - |
dc.subject.keyword | Recurrence | - |
dc.subject.keyword | Sclera/surgery* | - |
dc.subject.keyword | Silicone Elastomers* | - |
dc.subject.keyword | Suture Techniques | - |
dc.subject.keyword | Visual Acuity | - |
dc.contributor.alternativeName | Kim, Eun Hee | - |
dc.contributor.alternativeName | Kim, Jun O | - |
dc.contributor.alternativeName | Kim, Hye Young | - |
dc.contributor.alternativeName | Lee, Min Goo | - |
dc.contributor.affiliatedAuthor | Kim, Eun Hee | - |
dc.contributor.affiliatedAuthor | Kim, Jun O | - |
dc.contributor.affiliatedAuthor | Kim, Hye Young | - |
dc.contributor.affiliatedAuthor | Lee, Min Goo | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 283 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 1018 | - |
dc.citation.endPage | 1025 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, Vol.283(2) : 1018-1025, 2008 | - |
dc.identifier.rimsid | 54740 | - |
dc.type.rims | ART | - |
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