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Identification of antigen-presenting dendritic cells in mouse aorta and cardiac valves.

Authors
 Jae-Hoon Choi  ;  Yoonkyung Do  ;  Cheolho Cheong  ;  Hyein Koh  ;  Silvia B. Boscardin  ;  Yong-Seok Oh  ;  Leonia Bozzacco  ;  Christine Trumpfheller  ;  Chae Gyu Park  ;  Ralph M. Steinman 
Citation
 JOURNAL OF EXPERIMENTAL MEDICINE, Vol.206(3) : 497-505, 2009 
Journal Title
 JOURNAL OF EXPERIMENTAL MEDICINE 
ISSN
 0022-1007 
Issue Date
2009
MeSH
Animals ; Antigen Presentation/immunology* ; Antigens/immunology ; Aorta/cytology* ; Aorta/immunology* ; Bacterial Proteins/metabolism ; Biomarkers/metabolism ; CD11c Antigen/immunology ; Cell Membrane/immunology ; Cell Movement ; Cross-Priming/immunology ; Dendritic Cells/cytology* ; Dendritic Cells/immunology* ; Heart Valves/cytology* ; Heart Valves/immunology* ; Histocompatibility Antigens Class I/immunology ; Luminescent Proteins/metabolism ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Phenotype ; Recombinant Fusion Proteins/metabolism ; Sinus of Valsalva/cytology ; Sinus of Valsalva/immunology ; Spleen/cytology ; Spleen/immunology
Abstract
Presumptive dendritic cells (DCs) bearing the CD11c integrin and other markers have previously been identified in normal mouse and human aorta. We used CD11c promoter-enhanced yellow fluorescent protein (EYFP) transgenic mice to visualize aortic DCs and study their antigen-presenting capacity. Stellate EYFP(+) cells were readily identified in the aorta and could be double labeled with antibodies to CD11c and antigen-presenting major histocompatability complex (MHC) II products. The DCs proved to be particularly abundant in the cardiac valves and aortic sinus. In all aortic locations, the CD11c(+) cells localized to the subintimal space with occasional processes probing the vascular lumen. Aortic DCs expressed little CD40 but expressed low levels of CD1d, CD80, and CD86. In studies of antigen presentation, DCs selected on the basis of EYFP expression or binding of anti-CD11c antibody were as effective as DCs similarly selected from the spleen. In particular, the aortic DCs could cross-present two different protein antigens on MHC class I to CD8(+) TCR transgenic T cells. In addition, after intravenous injection, aortic DCs could capture anti-CD11c antibody and cross-present ovalbumin to T cells. These results indicate that bona fide DCs are a constituent of the normal aorta and cardiac valves.
Files in This Item:
T200906220.pdf Download
DOI
10.1084/jem.20082129
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Park, Chae Gyu(박채규) ORCID logo https://orcid.org/0000-0003-1906-1308
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/106077
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