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Identification of antigen-presenting dendritic cells in mouse aorta and cardiac valves.

DC Field Value Language
dc.contributor.author박채규-
dc.date.accessioned2015-04-24T17:47:29Z-
dc.date.available2015-04-24T17:47:29Z-
dc.date.issued2009-
dc.identifier.issn0022-1007-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/106077-
dc.description.abstractPresumptive dendritic cells (DCs) bearing the CD11c integrin and other markers have previously been identified in normal mouse and human aorta. We used CD11c promoter-enhanced yellow fluorescent protein (EYFP) transgenic mice to visualize aortic DCs and study their antigen-presenting capacity. Stellate EYFP(+) cells were readily identified in the aorta and could be double labeled with antibodies to CD11c and antigen-presenting major histocompatability complex (MHC) II products. The DCs proved to be particularly abundant in the cardiac valves and aortic sinus. In all aortic locations, the CD11c(+) cells localized to the subintimal space with occasional processes probing the vascular lumen. Aortic DCs expressed little CD40 but expressed low levels of CD1d, CD80, and CD86. In studies of antigen presentation, DCs selected on the basis of EYFP expression or binding of anti-CD11c antibody were as effective as DCs similarly selected from the spleen. In particular, the aortic DCs could cross-present two different protein antigens on MHC class I to CD8(+) TCR transgenic T cells. In addition, after intravenous injection, aortic DCs could capture anti-CD11c antibody and cross-present ovalbumin to T cells. These results indicate that bona fide DCs are a constituent of the normal aorta and cardiac valves.-
dc.description.statementOfResponsibilityopen-
dc.format.extent497~505-
dc.relation.isPartOfJOURNAL OF EXPERIMENTAL MEDICINE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHAntigen Presentation/immunology*-
dc.subject.MESHAntigens/immunology-
dc.subject.MESHAorta/cytology*-
dc.subject.MESHAorta/immunology*-
dc.subject.MESHBacterial Proteins/metabolism-
dc.subject.MESHBiomarkers/metabolism-
dc.subject.MESHCD11c Antigen/immunology-
dc.subject.MESHCell Membrane/immunology-
dc.subject.MESHCell Movement-
dc.subject.MESHCross-Priming/immunology-
dc.subject.MESHDendritic Cells/cytology*-
dc.subject.MESHDendritic Cells/immunology*-
dc.subject.MESHHeart Valves/cytology*-
dc.subject.MESHHeart Valves/immunology*-
dc.subject.MESHHistocompatibility Antigens Class I/immunology-
dc.subject.MESHLuminescent Proteins/metabolism-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred C57BL-
dc.subject.MESHMice, Transgenic-
dc.subject.MESHPhenotype-
dc.subject.MESHRecombinant Fusion Proteins/metabolism-
dc.subject.MESHSinus of Valsalva/cytology-
dc.subject.MESHSinus of Valsalva/immunology-
dc.subject.MESHSpleen/cytology-
dc.subject.MESHSpleen/immunology-
dc.titleIdentification of antigen-presenting dendritic cells in mouse aorta and cardiac valves.-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Life Science (의생명과학부)-
dc.contributor.googleauthorJae-Hoon Choi-
dc.contributor.googleauthorYoonkyung Do-
dc.contributor.googleauthorCheolho Cheong-
dc.contributor.googleauthorHyein Koh-
dc.contributor.googleauthorSilvia B. Boscardin-
dc.contributor.googleauthorYong-Seok Oh-
dc.contributor.googleauthorLeonia Bozzacco-
dc.contributor.googleauthorChristine Trumpfheller-
dc.contributor.googleauthorChae Gyu Park-
dc.contributor.googleauthorRalph M. Steinman-
dc.identifier.doi10.1084/jem.20082129-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01718-
dc.relation.journalcodeJ01409-
dc.identifier.eissn1540-9538-
dc.identifier.pmid19221394-
dc.contributor.alternativeNamePark, Chae Gyu-
dc.contributor.affiliatedAuthorPark, Chae Gyu-
dc.citation.volume206-
dc.citation.number3-
dc.citation.startPage497-
dc.citation.endPage505-
dc.identifier.bibliographicCitationJOURNAL OF EXPERIMENTAL MEDICINE, Vol.206(3) : 497-505, 2009-
dc.identifier.rimsid57024-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers

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