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구강상피세포의 냉동보관 방법에 따른 세포생존률 비교
DC Field | Value | Language |
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dc.contributor.author | 김의성 | - |
dc.contributor.author | 이승종 | - |
dc.contributor.author | 정한성 | - |
dc.date.accessioned | 2015-04-24T17:28:41Z | - |
dc.date.available | 2015-04-24T17:28:41Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 2234-7658 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/105473 | - |
dc.description.abstract | This study examined the influence of the storage methods on the viability of oral epithelial cells using conventional cell freezing storage, slow freezing preservation, rapid freezing preservation, and slow freezing preservation with a pressure of 2 Mpa or 3 Mpa. The cell viability was evaluated by cell counting, WST-1 and the clonogenic capacity after 6 days of freezing storage. After 6 days, the frozen cells were thawed rapidly, and the cell counting. WST-1, and clonogenic capacity values were measured and compared. 1. The results from cell counting demonstrated that conventional cryopreservation, slow freezing under a 2 Mpa pressure and slow freezing under a 3 Mpa pressure showed significantly higher values than slow freezing preservation and rapid freezing preservation (p < 0.05). 2. The results from the optical density by WST-1 demonstrated that slow freezing under a 2 Mpa pressure showed significantly higher values than slow freezing preservation and rapid freezing preservation (p<0.05). 3. The clonogenic capacity demonstrated that slow freezing under a 2 Mpa pressure showed significantly higher values than slow freezing preservation and rapid freezing preservation (p < 0.05). | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 491~499 | - |
dc.relation.isPartOf | Journal of Korean Academy of Operative Dentistry (대한치과보존학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | 구강상피세포의 냉동보관 방법에 따른 세포생존률 비교 | - |
dc.title.alternative | COMPARISON OF VIABILITY OF ORAL EPITHELIAL CELLS STORED BY DIFFERENT FREEZING METHODS | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral Biology (구강생물학) | - |
dc.contributor.googleauthor | 백도영 | - |
dc.contributor.googleauthor | 이승종 | - |
dc.contributor.googleauthor | 정한성 | - |
dc.contributor.googleauthor | 김의성 | - |
dc.identifier.doi | 10.5395/JKACD.2009.34.6.491 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00833 | - |
dc.contributor.localId | A02925 | - |
dc.contributor.localId | A03758 | - |
dc.relation.journalcode | J01488 | - |
dc.subject.keyword | WST-1 | - |
dc.subject.keyword | Cell counting | - |
dc.subject.keyword | Clonogenic capacity | - |
dc.subject.keyword | Slow freezing under pressure | - |
dc.subject.keyword | Slow freezing | - |
dc.subject.keyword | Rapid freezing | - |
dc.contributor.alternativeName | Kim, Eui Seong | - |
dc.contributor.alternativeName | Lee, Seung Jong | - |
dc.contributor.alternativeName | Jung, Han Sung | - |
dc.contributor.affiliatedAuthor | Kim, Eui Seong | - |
dc.contributor.affiliatedAuthor | Lee, Seung Jong | - |
dc.contributor.affiliatedAuthor | Jung, Han Sung | - |
dc.citation.volume | 34 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 491 | - |
dc.citation.endPage | 499 | - |
dc.identifier.bibliographicCitation | Journal of Korean Academy of Operative Dentistry (대한치과보존학회지), Vol.34(6) : 491-499, 2009 | - |
dc.identifier.rimsid | 44290 | - |
dc.type.rims | ART | - |
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