0 155

Cited 0 times in

Stroma-free mass production of clinical-grade red blood cells (RBCs) by using poloxamer 188 as an RBC survival enhancer

Authors
 Eun Jung Baek  ;  Han-Soo Kim  ;  Ju-Hye Kim  ;  Nahn Ju Kim  ;  Hyun Ok Kim 
Citation
 TRANSFUSION, Vol.49(11) : 2285-2295, 2009 
Journal Title
 TRANSFUSION 
ISSN
 0041-1132 
Issue Date
2009
MeSH
Antigens, CD34/metabolism ; Cell Culture Techniques/methods* ; Cell Separation/methods ; Cell Survival ; Erythrocytes/cytology* ; Erythrocytes/drug effects ; Erythrocytes/ultrastructure ; Fetal Blood/cytology ; Flow Cytometry ; Humans ; Microscopy, Electron, Scanning ; Microscopy, Electron, Transmission ; Plasma/cytology ; Poloxamer/pharmacology ; Stromal Cells/cytology*
Abstract
BACKGROUND: In vitro generation of red blood cells (RBCs) is an important alternative to donor RBCs. It was impossible, however, to generate a large quantity of RBCs due to necessity of supporting stromal cells or xenogeneic or human serum for in vitro culture, which had restrictions in safety, supplies, and expenses. In addition, the low viability of erythroblasts during terminal maturation in vitro required highly efficient production protocols. Here, we present a protocol for mass production of clinical-grade RBCs from cord blood (CB) CD34+ cells in stroma-free culture by using poloxamer 188 (P188), a polymer known to be cytoprotective against hydrodynamic stress. STUDY DESIGN AND METHODS: Umbilical CB CD34+ cells were cultured for 21 days in medium containing several cytokines, and roughly fibrin-eliminated CB derived plasma was added from Day 13. To enhance the final RBC production, P188 was added from Day 13 and the cell count and viability were compared with controls lacking P188. RESULTS: Erythroid expansion between Day 17 and Day 21 was significantly higher in cultures treated with P188, with a mean of 1.5 times and maximum of 3.6 times higher expansion than in controls lacking P188. The enhanced survival resulted from increased stability of the RBC membrane and decreased fragility. The erythroid cells were enucleated up to 95% and demonstrated hematologic variables similar to those of reticulocytes. CONCLUSION: This RBC production protocol is a simple stroma- and serum-free culture method ensuring enhanced viability of terminally mature erythroid cells and can be easily applicable to mass production of clinical-grade RBCs as well as erythropoiesis research.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/j.1537-2995.2009.02303.x/abstract
DOI
10.1111/j.1537-2995.2009.02303.x
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Han Soo(김한수)
Kim, Hyun Ok(김현옥) ORCID logo https://orcid.org/0000-0002-4964-1963
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/105389
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse