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Escherichia coli, Klebsiella pneumoniae 및 Proteus mirabilis에서 플라즈미드매개성 AmpC β-lactamase를 검출하는 3가지 표현형 검사법의 비교

Other Titles
 Comparison of 3 Phenotypic-detection Methods for Identifying Plasmid-mediated AmpC beta-lactamase-producing Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis Strains 
Authors
 Wookeun Lee  ;  Bochan Jung  ;  Seong Geun Hong  ;  Wonkeun Song  ;  Seok Hoon Jeong  ;  Kyungwon Lee  ;  Hyo-Sun Kwak 
Citation
 KOREAN JOURNAL OF LABORATORY MEDICINE , Vol.29(5) : 448-454, 2009 
Journal Title
 KOREAN JOURNAL OF LABORATORY MEDICINE 
ISSN
 1598-6535 
Issue Date
2009
MeSH
Anti-Bacterial Agents/pharmacology ; Bacterial Proteins/analysis* ; Cefotetan/pharmacology ; Cefoxitin/pharmacology ; Disk Diffusion Antimicrobial Tests/methods* ; Escherichia coli/genetics ; Escherichia coli/isolation & purification* ; Humans ; Klebsiella pneumoniae/genetics ; Klebsiella pneumoniae/isolation & purification* ; Phenotype ; Plasmids ; Proteus mirabilis/genetics ; Proteus mirabilis/isolation & purification* ; Sensitivity and Specificity ; beta-Lactamases/analysis*
Keywords
AmpC β-lactamase ; Boronic acid ; Hodge test ; Tris-EDTA disk test
Abstract
BACKGROUND: Plasmid-mediated AmpC beta-lactamases (PABLs) have been detected in the strains of Escherichia coli, Klebsiella spp., Proteus mirabilis, and Salmonella spp. PABLs may be difficult to detect and might interfere in the therapeutic and infection-control processes. Although several PABL-detection methods based on phenotypes have been reported, the Clinical and Laboratory Standards Institute currently does not recommend a routine detection method for PABLs. The aim of this study is to compare the performances of 3 phenotypic PABL detection methods. METHODS: Total 276 non-duplicated clinical isolates of E. coli (N=97), K. pneumoniae (N=136), and P. mirabilis (N=43) were collected from 14 hospitals in Korea between April and June 2007 in a non-consecutive and non-random manner. Multiplex PCR was performed to detect the PABL genes. Further, 3 phenotypic detection methods-cephamycin-Hodge test, Tris-EDTA (TE) disk test, and combination-disk test with 3-aminophenylboronic acid (BA)-were performed using cefoxitin and cefotetan disks. RESULTS: PABL genes were detected by multiplex PCR in 122/276 isolates, including 14/97 E. coli, 105/136 K. pneumoniae, and 3/43 P. mirabilis isolates. The combination-disk test with BA showed higher sensitivity (98.4%), specificity (92.2%), and efficiency (96.3%) than the cephamycin-Hodge (76.2%, 96.1%, and 88.6%, respectively) and the TE-disk (80.3%, 91.6%, and 87.9%, respectively) tests. CONCLUSIONS: The combination-disk test with BA is a simple, efficient, and interpretable test that can be applicable in clinical laboratories involved in the detection of PABLs in clinical isolates of E. coli, K. pneumoniae, and P. mirabilis.
Files in This Item:
T200903778.pdf Download
DOI
10.3343/kjlm.2009.29.5.448
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
Jeong, Seok Hoon(정석훈) ORCID logo https://orcid.org/0000-0001-9290-897X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/105375
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