Cited 16 times in
Escherichia coli, Klebsiella pneumoniae 및 Proteus mirabilis에서 플라즈미드매개성 AmpC β-lactamase를 검출하는 3가지 표현형 검사법의 비교
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이경원 | - |
dc.contributor.author | 정석훈 | - |
dc.date.accessioned | 2015-04-24T17:25:31Z | - |
dc.date.available | 2015-04-24T17:25:31Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 1598-6535 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/105375 | - |
dc.description.abstract | BACKGROUND: Plasmid-mediated AmpC beta-lactamases (PABLs) have been detected in the strains of Escherichia coli, Klebsiella spp., Proteus mirabilis, and Salmonella spp. PABLs may be difficult to detect and might interfere in the therapeutic and infection-control processes. Although several PABL-detection methods based on phenotypes have been reported, the Clinical and Laboratory Standards Institute currently does not recommend a routine detection method for PABLs. The aim of this study is to compare the performances of 3 phenotypic PABL detection methods. METHODS: Total 276 non-duplicated clinical isolates of E. coli (N=97), K. pneumoniae (N=136), and P. mirabilis (N=43) were collected from 14 hospitals in Korea between April and June 2007 in a non-consecutive and non-random manner. Multiplex PCR was performed to detect the PABL genes. Further, 3 phenotypic detection methods-cephamycin-Hodge test, Tris-EDTA (TE) disk test, and combination-disk test with 3-aminophenylboronic acid (BA)-were performed using cefoxitin and cefotetan disks. RESULTS: PABL genes were detected by multiplex PCR in 122/276 isolates, including 14/97 E. coli, 105/136 K. pneumoniae, and 3/43 P. mirabilis isolates. The combination-disk test with BA showed higher sensitivity (98.4%), specificity (92.2%), and efficiency (96.3%) than the cephamycin-Hodge (76.2%, 96.1%, and 88.6%, respectively) and the TE-disk (80.3%, 91.6%, and 87.9%, respectively) tests. CONCLUSIONS: The combination-disk test with BA is a simple, efficient, and interpretable test that can be applicable in clinical laboratories involved in the detection of PABLs in clinical isolates of E. coli, K. pneumoniae, and P. mirabilis. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 448~454 | - |
dc.relation.isPartOf | KOREAN JOURNAL OF LABORATORY MEDICINE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Anti-Bacterial Agents/pharmacology | - |
dc.subject.MESH | Bacterial Proteins/analysis* | - |
dc.subject.MESH | Cefotetan/pharmacology | - |
dc.subject.MESH | Cefoxitin/pharmacology | - |
dc.subject.MESH | Disk Diffusion Antimicrobial Tests/methods* | - |
dc.subject.MESH | Escherichia coli/genetics | - |
dc.subject.MESH | Escherichia coli/isolation & purification* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Klebsiella pneumoniae/genetics | - |
dc.subject.MESH | Klebsiella pneumoniae/isolation & purification* | - |
dc.subject.MESH | Phenotype | - |
dc.subject.MESH | Plasmids | - |
dc.subject.MESH | Proteus mirabilis/genetics | - |
dc.subject.MESH | Proteus mirabilis/isolation & purification* | - |
dc.subject.MESH | Sensitivity and Specificity | - |
dc.subject.MESH | beta-Lactamases/analysis* | - |
dc.title | Escherichia coli, Klebsiella pneumoniae 및 Proteus mirabilis에서 플라즈미드매개성 AmpC β-lactamase를 검출하는 3가지 표현형 검사법의 비교 | - |
dc.title.alternative | Comparison of 3 Phenotypic-detection Methods for Identifying Plasmid-mediated AmpC beta-lactamase-producing Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis Strains | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학) | - |
dc.contributor.googleauthor | Wookeun Lee | - |
dc.contributor.googleauthor | Bochan Jung | - |
dc.contributor.googleauthor | Seong Geun Hong | - |
dc.contributor.googleauthor | Wonkeun Song | - |
dc.contributor.googleauthor | Seok Hoon Jeong | - |
dc.contributor.googleauthor | Kyungwon Lee | - |
dc.contributor.googleauthor | Hyo-Sun Kwak | - |
dc.identifier.doi | 10.3343/kjlm.2009.29.5.448 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02649 | - |
dc.contributor.localId | A03619 | - |
dc.relation.journalcode | J02044 | - |
dc.identifier.pmid | 19893354 | - |
dc.subject.keyword | AmpC β-lactamase | - |
dc.subject.keyword | Boronic acid | - |
dc.subject.keyword | Hodge test | - |
dc.subject.keyword | Tris-EDTA disk test | - |
dc.contributor.alternativeName | Lee, Kyung Won | - |
dc.contributor.alternativeName | Jeong, Seok Hoon | - |
dc.contributor.affiliatedAuthor | Lee, Kyung Won | - |
dc.contributor.affiliatedAuthor | Jeong, Seok Hoon | - |
dc.citation.volume | 29 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 448 | - |
dc.citation.endPage | 454 | - |
dc.identifier.bibliographicCitation | KOREAN JOURNAL OF LABORATORY MEDICINE , Vol.29(5) : 448-454, 2009 | - |
dc.identifier.rimsid | 51644 | - |
dc.type.rims | ART | - |
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