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Role of NtrC-regulated exopolysaccharides in the biofilm formation and pathogenic interaction of Vibrio vulnificus. Kim HS, Park SJ, Lee KH

Authors
 Han-Suk Kim  ;  Soon-Jung Park  ;  Kyu-Ho Lee 
Citation
 MOLECULAR MICROBIOLOGY, Vol.74(2) : 436-453, 2009 
Journal Title
MOLECULAR MICROBIOLOGY
ISSN
 0950-382X 
Issue Date
2009
MeSH
Animals ; Bacterial Adhesion ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism* ; Biofilms/growth & development* ; Cell Line ; Female ; Gene Expression Regulation, Bacterial ; Genes, Bacterial ; Genetic Complementation Test ; Humans ; Mice ; Mice, Inbred ICR ; Multigene Family ; Mutation ; Polysaccharides, Bacterial/biosynthesis* ; Transcription Factors/genetics ; Transcription Factors/metabolism* ; Vibrio vulnificus/genetics* ; Vibrio vulnificus/growth & development ; Vibrio vulnificus/metabolism ; Vibrio vulnificus/pathogenicity
Abstract
Vibrio vulnificus has been shown to require a global transcription factor, NtrC for mature biofilm development via controlling the biosyntheses of lipopolysaccharide and exopolysaccharide (EPS). Biofilm formation and EPS production were dramatically increased in a medium including a tricarboxylic acid cycle-intermediate as a carbon source. These phenotypes required functional NtrC and were abolished by the addition of ammonium chloride. During the initial stage of biofilm formation, both expression of the ntrC gene and the cellular content of NtrC protein increased. Thus, the regulatory roles of NtrC in EPS biosynthesis were studied with three gene clusters for EPS biosyntheses. Transcriptions of the three clusters were positively controlled by NtrC and showed maximal expression at the early stage of biofilm development. Mutants deficient in one of the genes (VV1_2661, VV2_1579 and VV1_2305) in each cluster showed decreased production of EPS, attenuated ability to form biofilm and lowered cytoadherence to human epithelial cells. However, mutations in VV2_1579 and VV1_2305 resulted in lower cytotoxicity to human cells and mortality to mice than the mutation in VV1_2661. These results demonstrate that NtrC-regulated EPS are crucial in biofilm formation of V. vulnificus, and some EPS components play important roles in interacting with hosts.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2009.06875.x/abstract
DOI
10.1111/j.1365-2958.2009.06875.x
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Tropica Medicine (열대의학교실) > 1. Journal Papers
Yonsei Authors
Park, Soon Jung(박순정) ORCID logo https://orcid.org/0000-0002-0423-1944
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/105349
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