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사람 양수에서 호흡기세포의 분리

Other Titles
 Isolation and Identification of Respiratory Cells from Human Amniotic Fluid 
Authors
 Eun Jung Kim  ;  Yong Won Park  ;  Young Han Kim  ;  Yu Seun Kim  ;  Jung-Tak Oh 
Citation
 Journal of the Korean Association of Pediatric Surgeons (소아외과), Vol.15(1) : 1-10, 2009 
Journal Title
 Journal of the Korean Association of Pediatric Surgeons (소아외과) 
ISSN
 1225-9047 
Issue Date
2009
Keywords
Amniotic fluid ; Respiratory cell ; Isolation ; Human
Abstract
Recently, amniotic fluid has gained attention as one of the potential sources for cell therapy and tissue engineering because it has characteristics of multipotent stem cells. However, current knowledge about what types of cells are naturally found in amniotic fluid is still limited. In this study, we aimed to investigate whether human amniotic fluid contains cells that have characteristics of respiratory cells. Samples of human amniotic fluid (5 mL per sample) obtained from amniocenteses were cultured with small airway growth medium (SAGM). Cells were grown until the third passage and the presence of type II alveolar cells were characterized by inverted microscopy, immunofluorescence, and reverse transcription polymerase chain reaction (RT-PCR). On inverted microscopy, cultured cells showed typical polygonal and cobblestone-like epithelial morphology. The morphology of cells was not changed after selection and passing. Immunofluorescence analysis demonstrated that the isolated cells stained positive for surfactant protein C (SPC), specific marker for type II alveolar cells. Cells also stained positive for TTF-1 protein but negative for CD 31 and vimentin. RT-PCR analysis of cells showed expression of SPC mRNA. This study has demonstrated that respiratory cells can be isolated and identified from human amniotic fluid cultured in SAGM medium. Our results may provide the basis for further investigations of amniotic fluid
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Young Han(김영한) ORCID logo https://orcid.org/0000-0003-0645-6028
Kim, Yu Seun(김유선) ORCID logo https://orcid.org/0000-0002-5105-1567
Park, Yong Won(박용원)
Oh, Jung Tak(오정탁)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/105223
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