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사람 양수에서 호흡기세포의 분리

DC FieldValueLanguage
dc.contributor.author김영한-
dc.contributor.author김유선-
dc.contributor.author박용원-
dc.contributor.author오정탁-
dc.date.accessioned2015-04-24T17:20:42Z-
dc.date.available2015-04-24T17:20:42Z-
dc.date.issued2009-
dc.identifier.issn1225-9047-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/105223-
dc.description.abstractRecently, amniotic fluid has gained attention as one of the potential sources for cell therapy and tissue engineering because it has characteristics of multipotent stem cells. However, current knowledge about what types of cells are naturally found in amniotic fluid is still limited. In this study, we aimed to investigate whether human amniotic fluid contains cells that have characteristics of respiratory cells. Samples of human amniotic fluid (5 mL per sample) obtained from amniocenteses were cultured with small airway growth medium (SAGM). Cells were grown until the third passage and the presence of type II alveolar cells were characterized by inverted microscopy, immunofluorescence, and reverse transcription polymerase chain reaction (RT-PCR). On inverted microscopy, cultured cells showed typical polygonal and cobblestone-like epithelial morphology. The morphology of cells was not changed after selection and passing. Immunofluorescence analysis demonstrated that the isolated cells stained positive for surfactant protein C (SPC), specific marker for type II alveolar cells. Cells also stained positive for TTF-1 protein but negative for CD 31 and vimentin. RT-PCR analysis of cells showed expression of SPC mRNA. This study has demonstrated that respiratory cells can be isolated and identified from human amniotic fluid cultured in SAGM medium. Our results may provide the basis for further investigations of amniotic fluid-
dc.description.statementOfResponsibilityopen-
dc.format.extent1~10-
dc.relation.isPartOfJournal of the Korean Association of Pediatric Surgeons (소아외과)-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.title사람 양수에서 호흡기세포의 분리-
dc.title.alternativeIsolation and Identification of Respiratory Cells from Human Amniotic Fluid-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Obstetrics & Gynecology (산부인과학)-
dc.contributor.googleauthorEun Jung Kim-
dc.contributor.googleauthorYong Won Park-
dc.contributor.googleauthorYoung Han Kim-
dc.contributor.googleauthorYu Seun Kim-
dc.contributor.googleauthorJung-Tak Oh-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00730-
dc.contributor.localIdA00785-
dc.contributor.localIdA01581-
dc.contributor.localIdA02397-
dc.relation.journalcodeJ01808-
dc.subject.keywordAmniotic fluid-
dc.subject.keywordRespiratory cell-
dc.subject.keywordIsolation-
dc.subject.keywordHuman-
dc.contributor.alternativeNameKim, Young Han-
dc.contributor.alternativeNameKim, Yu Seun-
dc.contributor.alternativeNamePark, Yong Won-
dc.contributor.alternativeNameOh, Jung Tak-
dc.contributor.affiliatedAuthorKim, Young Han-
dc.contributor.affiliatedAuthorKim, Yu Seun-
dc.contributor.affiliatedAuthorPark, Yong Won-
dc.contributor.affiliatedAuthorOh, Jung Tak-
dc.contributor.affiliatedAuthor오정탁-
dc.citation.volume15-
dc.citation.number1-
dc.citation.startPage1-
dc.citation.endPage10-
dc.identifier.bibliographicCitationJournal of the Korean Association of Pediatric Surgeons (소아외과), Vol.15(1) : 1-10, 2009-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers

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