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Sequence diversity of the Bla g 4 cockroach allergen, homologous to lipocalins, from Blattella germanica

Authors
 Kyoung Yong Jeong  ;  Myung-hee Yi  ;  Kyoung-jin Jeong  ;  Haeseok Lee  ;  Chein-Soo Hong  ;  Tai-Soon Yong 
Citation
 INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, Vol.148(4) : 339-345, 2009 
Journal Title
 INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY 
ISSN
 1018-2438 
Issue Date
2009
MeSH
Allergens/chemistry* ; Allergens/genetics* ; Allergens/immunology ; Amino Acid Sequence ; Animals ; Antibodies/immunology ; Antigens, Plant ; Blattellidae/chemistry ; Blattellidae/genetics* ; Cloning, Molecular ; DNA, Complementary/genetics ; Female ; Genetic Variation* ; Insect Proteins/chemistry* ; Insect Proteins/genetics* ; Insect Proteins/immunology ; Lipocalins/genetics ; Male ; Mice ; Mice, Inbred BALB C ; Molecular Sequence Data ; Sequence Homology, Amino Acid
Keywords
Bla g 4 ; Cockroach ; Polymorphism ; RNA editing
Abstract
BACKGROUND: The cockroach allergen Bla g 4, a putative lipocalin, is known to exhibit frequent sequence variations. However, the previously reported cDNA sequences are truncated at the N terminus. This study was undertaken to investigate the mechanisms by which these sequence variations are generated. METHODS: Rapid amplification of cDNA ends PCR and RT-PCR were performed to obtain the full sequence of the Bla g 4 cDNA, and PCR was also used to clone the Bla g 4 genomic DNA. In addition, Bla g 4 protein variants were analyzed by two-dimensional gel electrophoresis. RESULTS: Nine additional amino acid residues at the N terminus of Bla g 4 were identified, and 2 genes encoding Bla g 4, both of which consisted of 5 exons, were cloned. Examination of 34 clones of Bla g 4 cDNA obtained by RT-PCR revealed 14 variants. In particular, Bla g 4 sequences showed frequent clusters of variations in residues 38-45, 61-82 and 144-163. Differences in cDNA sequences may imply that RNA sequences are edited after transcription. More than 10 spots were identified between pH 5 and 7 upon two-dimensional gel electrophoresis, indicating that multiple variants of Bla g 4 are produced at the protein level. CONCLUSIONS: Genetic polymorphisms among individual cockroaches, the existence of multiple genes and sequence variations caused by RNA editing produce sequence diversity of Bla g 4, which may influence its allergenicity. The sequence information obtained in this study will be helpful for the standardization of the cockroach allergen and thereby aid in the development of diagnostics and immunotherapeutics
Full Text
http://www.karger.com/Article/FullText/170388
DOI
10.1159/000170388
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Institute of Allergy (알레르기연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
Jeong, Kyoung Yong(정경용) ORCID logo https://orcid.org/0000-0001-9887-1426
Hong, Chein Soo(홍천수)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/104810
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