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Activation of epithelial sodium channel in human middle ear epithelial cells by dexamethasone

Authors
 Eun Jin Son  ;  Sung Hunh Kim  ;  Hun Yi Park  ;  Su Jin Kim  ;  Joo-Heon Yoon  ;  Hyun Pil Chung  ;  Jae Young Choi 
Citation
 EUROPEAN JOURNAL OF PHARMACOLOGY, Vol.602(2-3) : 383-387, 2009 
Journal Title
 EUROPEAN JOURNAL OF PHARMACOLOGY 
ISSN
 0014-2999 
Issue Date
2009
MeSH
Absorption/drug effects ; Body Fluids/drug effects ; Body Fluids/metabolism ; Cells, Cultured ; Dexamethasone/pharmacology* ; Ear, Middle/cytology* ; Electric Conductivity ; Epithelial Cells/drug effects* ; Epithelial Cells/metabolism* ; Epithelial Sodium Channel Agonists* ; Epithelial Sodium Channels/genetics ; Epithelial Sodium Channels/metabolism ; Gene Expression Regulation/drug effects ; Humans ; RNA, Messenger/genetics ; RNA, Messenger/metabolism
Keywords
Dexamethasone ; Otitis media with effusion ; Fluid absorption ; Middle ear ; Epithelial sodium channel
Abstract
The middle ear epithelium functions to maintain a fluid-free middle ear cavity. Dysfunction of the middle ear epithelial ion and fluid transport is implicated in the pathogenesis of fluid collection in the middle ear cavity, characteristic of otitis media with effusion. The efficacy of steroid therapy for the treatment of otitis media with effusion remains controversial, and postulated modulation of transepithelial transport function in middle ear epithelia has yet to be demonstrated. The effect of dexamethasone on Na(+) transport and fluid absorption capacity was investigated in cultured normal human middle ear epithelial (NHMEE) cells. Dexamethasone produced a significant increase in amiloride-sensitive short-circuit current (Isc). Dexamethasone significantly increased expression levels of mRNAs and proteins of Epithelial Sodium Channel (ENaC)-alpha and -beta subunits. In addition, the ENaC-dependent fluid absorption was significantly increased after dexamethasone treatment. In summary, we have shown that dexamethasone stimulates ENaC activity and ENaC-dependent fluid absorption in NHMEE cells. These findings suggest glucocorticosteroids may be beneficial in treatment of otitis media with effusion by stimulating Na(+) transport and fluid clearance in the middle ear epithelia
Full Text
http://www.sciencedirect.com/science/article/pii/S001429990801128X
DOI
10.1016/j.ejphar.2008.11.012
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sung Huhn(김성헌)
Yoon, Joo Heon(윤주헌)
Choi, Jae Young(최재영) ORCID logo https://orcid.org/0000-0001-9493-3458
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/104760
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