7 220

Cited 13 times in

Activation of epithelial sodium channel in human middle ear epithelial cells by dexamethasone

DC FieldValueLanguage
dc.contributor.author김성헌-
dc.contributor.author윤주헌-
dc.contributor.author최재영-
dc.date.accessioned2015-04-24T17:06:02Z-
dc.date.available2015-04-24T17:06:02Z-
dc.date.issued2009-
dc.identifier.issn0014-2999-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/104760-
dc.description.abstractThe middle ear epithelium functions to maintain a fluid-free middle ear cavity. Dysfunction of the middle ear epithelial ion and fluid transport is implicated in the pathogenesis of fluid collection in the middle ear cavity, characteristic of otitis media with effusion. The efficacy of steroid therapy for the treatment of otitis media with effusion remains controversial, and postulated modulation of transepithelial transport function in middle ear epithelia has yet to be demonstrated. The effect of dexamethasone on Na(+) transport and fluid absorption capacity was investigated in cultured normal human middle ear epithelial (NHMEE) cells. Dexamethasone produced a significant increase in amiloride-sensitive short-circuit current (Isc). Dexamethasone significantly increased expression levels of mRNAs and proteins of Epithelial Sodium Channel (ENaC)-alpha and -beta subunits. In addition, the ENaC-dependent fluid absorption was significantly increased after dexamethasone treatment. In summary, we have shown that dexamethasone stimulates ENaC activity and ENaC-dependent fluid absorption in NHMEE cells. These findings suggest glucocorticosteroids may be beneficial in treatment of otitis media with effusion by stimulating Na(+) transport and fluid clearance in the middle ear epithelia-
dc.description.statementOfResponsibilityopen-
dc.format.extent383~387-
dc.relation.isPartOfEuropean Journal of Pharmacology-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAbsorption/drug effects-
dc.subject.MESHBody Fluids/drug effects-
dc.subject.MESHBody Fluids/metabolism-
dc.subject.MESHCells, Cultured-
dc.subject.MESHDexamethasone/pharmacology*-
dc.subject.MESHEar, Middle/cytology*-
dc.subject.MESHElectric Conductivity-
dc.subject.MESHEpithelial Cells/drug effects*-
dc.subject.MESHEpithelial Cells/metabolism*-
dc.subject.MESHEpithelial Sodium Channel Agonists*-
dc.subject.MESHEpithelial Sodium Channels/genetics-
dc.subject.MESHEpithelial Sodium Channels/metabolism-
dc.subject.MESHGene Expression Regulation/drug effects-
dc.subject.MESHHumans-
dc.subject.MESHRNA, Messenger/genetics-
dc.subject.MESHRNA, Messenger/metabolism-
dc.titleActivation of epithelial sodium channel in human middle ear epithelial cells by dexamethasone-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Otorhinolaryngology (이비인후과학)-
dc.contributor.googleauthorEun Jin Son-
dc.contributor.googleauthorSung Hunh Kim-
dc.contributor.googleauthorHun Yi Park-
dc.contributor.googleauthorSu Jin Kim-
dc.contributor.googleauthorJoo-Heon Yoon-
dc.contributor.googleauthorHyun Pil Chung-
dc.contributor.googleauthorJae Young Choi-
dc.identifier.doi10.1016/j.ejphar.2008.11.012-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA04173-
dc.contributor.localIdA00589-
dc.contributor.localIdA02604-
dc.relation.journalcodeJ00842-
dc.identifier.pmid19028490-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S001429990801128X-
dc.subject.keywordDexamethasone-
dc.subject.keywordOtitis media with effusion-
dc.subject.keywordFluid absorption-
dc.subject.keywordMiddle ear-
dc.subject.keywordEpithelial sodium channel-
dc.contributor.alternativeNameKim, Sung Huhn-
dc.contributor.alternativeNameYoon, Joo Heon-
dc.contributor.alternativeNameChoi, Jae Young-
dc.contributor.affiliatedAuthorChoi, Jae Young-
dc.contributor.affiliatedAuthorKim, Sung Huhn-
dc.contributor.affiliatedAuthorYoon, Joo Heon-
dc.citation.volume602-
dc.citation.number2-3-
dc.citation.startPage383-
dc.citation.endPage387-
dc.identifier.bibliographicCitationEuropean Journal of Pharmacology, Vol.602(2-3) : 383-387, 2009-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.