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Odontoblast TRP channels and thermo/mechanical transmission.

Authors
 A.R. Son  ;  Y.M. Yang  ;  J.H. Hong  ;  S.I. Lee  ;  Y. Shibukawa  ;  D.M. Shin 
Citation
 JOURNAL OF DENTAL RESEARCH, Vol.88(11) : 1014-1019, 2009 
Journal Title
 JOURNAL OF DENTAL RESEARCH 
ISSN
 0022-0345 
Issue Date
2009
MeSH
Animals ; Animals, Newborn ; Ankyrin Repeat ; Calcium Channels/analysis ; Calcium Signaling/physiology ; Cells, Cultured ; Cold Temperature ; Dental Pulp/cytology ; Hot Temperature ; Mechanoreceptors/physiology ; Mechanotransduction, Cellular/physiology* ; Mice ; Mice, Inbred ICR ; Odontoblasts/physiology* ; Osmosis ; Signal Transduction/physiology* ; TRPA1 Cation Channel ; TRPM Cation Channels/analysis ; TRPV Cation Channels/analysis ; Thermosensing/physiology* ; Transient Receptor Potential Channels/analysis ; Transient Receptor Potential Channels/physiology*
Keywords
TRP channels ; odontoblasts ; heat ; mechanosensitive
Abstract
Odontoblasts function as mechanosensory receptors because of the expression of mechanosensitive channels in these cells. However, it is unclear if odontoblasts direct the signal transmission evoked by heat/cold or osmotic changes. This study investigated the effects of heat/cold or osmotic changes on calcium signaling and the functional expression of the thermo/mechanosensitive transient receptor potential (TRP) channels in primary cultured mouse odontoblastic cells, with the use of RT-PCR, fluorometric calcium imaging, and electrophysiology. TRPV1, TRPV2, TRPV3, TRPV4, and TRPM3 mRNA was expressed, but TRPM8 and TRPA1 mRNA was not. The receptor-specific stimulation of TRPV1-3 (heat-sensing receptors) and TRPV4/ TRPM3 (mechanic receptors) caused increases in the intracellular calcium concentration. Moreover, the channel activities of TRPV1-4 and TRPM3 were confirmed by a whole-cell patch-clamp technique. These results suggest that primary cultured mouse odontoblasts express heat/mechanosensitive TRP channels and play a role in the underlying mechanisms of thermo/mechanosensitive sensory transmission
Full Text
http://jdr.sagepub.com/content/88/11/1014.long
DOI
10.1177/0022034509343413
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Son, Seung Hwa(손승화)
Shin, Dong Min(신동민) ORCID logo https://orcid.org/0000-0001-6042-0435
Lee, Syng Ill(이승일)
Hong, Jeong Hee(홍정희)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/104744
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