Preparation of PLGA microparticles by a double emulsion solvent technique for sustained release of (-)-epigallocatechin gallate (EGCG) and their growth inhibitory effect on rat aortic smooth muscle cells

Abstract

In this study, we fabricated porous PLGA microparticles loaded with EGCG by a modified water-in-oil-in-water (W/ O/W) double emulsion solvent evaporation method. The physicochemical properties of the EGCG-loaded PLGA microparticles were studied using scanning electron microscopy (SEM), Fourier transformed-infrared (FT-IR) spectroscopy and in vitro release measurements. A remarkable burst effect was observed on first stage however the released amount was reduced largely with time. This result showed that the release of EGCG increased by its loading amount. Also, EGCG - loaded PLGA particles inhibited the growth of rat aortic smooth muslce cells (RASMC). This study demonstrates that PLGA microparticle system is potential as an efficient EGCG carrier in medical therapy.