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Induction of heme oxygenase-1 protects against podocyte apoptosis under diabetic conditions.

 Sang Choel Lee  ;  Seung Hyeok Han  ;  Jin Ji Li  ;  Sun Ha Lee  ;  Dong-Sub Jung  ;  Seung-Jae Kwak  ;  Seung Hye Kim  ;  Dong Ki Kim  ;  Tae-Hyun Yoo  ;  Jin Hyun Kim  ;  Se-Ho Chang  ;  Dae Suk Han  ;  Shin-Wook Kang 
 Kidney International, Vol.76(8) : 838-848, 2009 
Journal Title
 Kidney International 
Issue Date
Animals ; Apoptosis*/drug effects ; Caspase 3/metabolism ; Cells, Cultured ; Diabetes Mellitus, Experimental/complications* ; Diabetes Mellitus, Experimental/enzymology ; Diabetes Mellitus, Experimental/pathology ; Diabetic Nephropathies/enzymology ; Diabetic Nephropathies/etiology ; Diabetic Nephropathies/pathology ; Diabetic Nephropathies/prevention & control* ; Enzyme Inhibitors/pharmacology ; Female ; Glucose/metabolism* ; Heme Oxygenase (Decyclizing)/metabolism ; Heme Oxygenase-1/antagonists & inhibitors ; Heme Oxygenase-1/genetics ; Heme Oxygenase-1/metabolism* ; Hemin/pharmacology ; Hypoxia-Inducible Factor 1/metabolism ; Male ; Membrane Proteins/metabolism ; Mice ; Podocytes/drug effects ; Podocytes/enzymology* ; Podocytes/pathology ; Proto-Oncogene Proteins c-bcl-2/metabolism ; Protoporphyrins/pharmacology ; RNA Interference ; Rats ; Rats, Sprague-Dawley ; Up-Regulation ; bcl-2-Associated X Protein/metabolism
apoptosis ; diabetic nephropathy ; heme oxygenase-1 ; high glucose ; podocyte
Heme oxygenase-1 (HO-1) is an anti-oxidant enzyme normally upregulated in response to oxidant injury. Here we determined the role of HO-1 in podocyte apoptosis in glomeruli of streptozotocin-treated rats and in immortalized mouse podocytes cultured in media containing normal or high glucose. HO-1 expression, its activity, the ratio of Bax/Bcl-2 protein, and active caspase-3 fragments were all significantly higher in isolated glomeruli of diabetic rats and in high glucose-treated podocytes. These increases were inhibited by zinc protoporphyrin treatment of the rats or by HO-1 siRNA treatment of the podocytes in culture. The number of apoptotic cells was also significantly increased in the glomeruli of diabetic rats and in high glucose-treated podocytes. Inhibition of HO-1 accentuated the increase in apoptotic cells both in vivo and in vitro. Our findings suggest that HO-1 expression protects against podocyte apoptosis under diabetic conditions
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1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
Yonsei Authors
Kang, Shin Wook(강신욱) ORCID logo https://orcid.org/0000-0002-5677-4756
Kwak, Seung Jae(곽승재)
Kim, Dong Ki(김동기)
Kim, Seung Hye(김승혜)
Yoo, Tae Hyun(유태현) ORCID logo https://orcid.org/0000-0002-9183-4507
Lee, Sun Ha(이순하)
Jung, Dong Sub(정동섭)
Han, Dae Suk(한대석)
Han, Seung Jin(한승진)
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