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ABO 혈액형 및 D 혈액형 판정용 시약의 품질관리용 동결 적혈구 표준품 확립

Other Titles
 Development of Cryopreserved Red Blood Cell Panels for Verifying ABO and D Blood Grouping Reagents 
 송성욱  ;  최종현  ;  김신영  ;  김현옥  ;  민혜경  ;  김재옥  ;  신원 
 Korean Journal of Blood Transfusion (대한수혈학회지), Vol.20(1) : 46-54, 2009 
Journal Title
 Korean Journal of Blood Transfusion (대한수혈학회지) 
Issue Date
Blood grouping reagent ; High glycerol method ; Cryopreserved red blood cell
Background: ABO blood grouping reagent verification is essential to ascertain safe blood transfusions. However, the research use of donated blood products has been hampered in Korea by the blood transfusion law and management policies. In this study, we developed cryopreserved red blood cell (RBC) panels utilizing the high glycerol method to verify the ABO and D blood grouping reagents. In addition, we evaluated the stability of ABO and D antigenicity. Methods: Fresh blood was frozen by the high glycerol method, aliquoted and cryopreserved in 2 mL cryotubes. Twenty-four vials of bloods with types A (n=5), B (n=5), AB (n=4) and O (n=10) for ABO RBC panels, and eleven vials of blood types D positive (n=5), D negative (n=5) and D weak (n=1) for D RBC panels were established. Potency, avidity and specificity tests were carried out with four different commercial ABO and D blood grouping reagents. Results: The potency of cryopreserved RBCs after thawing showed no statistical difference compared with pre-freezing RBCs. Avidity time measurements were 5 seconds in ABO blood and 20 seconds in D positive blood. Specificity test uniformly showed 100% specificity. When thawed RBCs were stored at 4oC for 7 days, the potency test measured at intervals of 2 days showed no variation. Conclusion: Cryopreserved RBC panels produced by the high glycerol method showed excellent results in stability test with reagents produced by manufacturers in Korea. Therefore, these panels can be utilized as a reliable method of verifying blood grouping reagents.
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1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sin Young(김신영) ORCID logo https://orcid.org/0000-0002-2609-8945
Kim, Hyun Ok(김현옥) ORCID logo https://orcid.org/0000-0002-4964-1963
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