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Human anti-alpha-enolase antibody in sera from patients with Behçet's disease and rheumatologic disorders

 J.H. Lee  ;  S.B. Cho  ;  D. Bang  ;  S.H. Oh  ;  K.-J. Ahn  ;  J. Kim  ;  Y.-B. Park  ;  S.-K. Lee  ;  K.H. Lee 
 CLINICAL AND EXPERIMENTAL RHEUMATOLOGY, Vol.27(2 suppl 53) : 63-66, 2009 
Journal Title
Issue Date
Adult ; Autoantibodies/blood* ; Behcet Syndrome/complications ; Behcet Syndrome/enzymology* ; Behcet Syndrome/immunology ; Biomarkers/blood ; Case-Control Studies ; Female ; Humans ; Immunoglobulin M/blood* ; Male ; Middle Aged ; Phosphopyruvate Hydratase/immunology* ; Rheumatic Diseases/enzymology* ; Rheumatic Diseases/immunology ; Vasculitis/complications ; Vasculitis/enzymology ; Vasculitis/immunology
Behçet’s disease ; enolase ; systemic lupus erythematosus ; dermatomyositis ; rheumatoid arthritis ; Takayasu’s arteritis
OBJECTIVE: alpha-Enolase is a target antigen of IgM-type anti-endothelial cell antibody in patients with Behçet's disease (BD). The objective of this study was to assess the reactivity of serum anti-alpha-enolase antibodies in BD and in other rheumatologic diseases, and to evaluate the clinical significance of serum anti-alpha-enolase antibodies in BD. METHODS: Enzyme-linked immunosorbent assay (ELISA) and immunoblotting were used to examine serum samples from patients with BD (n=100), systemic lupus erythematosus (SLE) (n=50), systemic sclerosis (n=21), rheumatoid arthritis (RA) (n=20), Takayasu's arteritis (n=20), dermatomyositis (n=17), mixed connective tissue disease (MCTD) (n=11), and samples from healthy volunteer donors (n=23). The medical records of patients with BD were reviewed to investigate their clinical characteristics. RESULTS: Specific positive signals against recombinant human alpha-enolase were detected by IgM ELISA of serum samples from 56 of the 100 BD patients (56.0%), 24 of the 50 SLE patients (48.0%), 15 of the 21 systemic sclerosis patients (71.4%), 13 of the 20 RA patients (65.0%), 10 of the 20 Takayasu's arteritis patients (50.0%), 9 of the 17 dermatomyositis patients (52.9%), and 5 of the 11 MCTD patients (45.5%). The number of BD patients with vascular lesions was significantly higher in the anti-alpha-enolase antibody positive group than in the negative group (p=0.027). CONCLUSION: We demonstrated the reactivities of serum anti-alpha-enolase antibodies in BD and other rheumatologic diseases with moderate specificity and also found that serum anti-alpha-enolase antibodies in BD can be associated with vascular system involvement
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1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Park, Yong Beom(박용범)
Bang, Dong Sik(방동식)
Ahn, Keun Jae(안근재)
Oh, Sang Ho(오상호) ORCID logo https://orcid.org/0000-0002-4477-1400
Lee, Kwang Hoon(이광훈)
Lee, Soo Kon(이수곤)
Lee, Ju Hee(이주희) ORCID logo https://orcid.org/0000-0002-1739-5956
Cho, Sung Bin(조성빈)
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