The predictive role of E-cadherin and androgen receptor on in vitro chemosensitivity in triple-negative breast Cancer.

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MeSH: Adenocarcinoma/drug therapy ; Adenocarcinoma/metabolism ; Adenocarcinoma/mortality ; Adenosine Triphosphate/metabolism ; Adult ; Aged ; Antineoplastic Agents/therapeutic use* ; Biomarkers, Tumor/metabolism* ; Breast Neoplasms/drug therapy* ; Breast Neoplasms/metabolism* ; Breast Neoplasms/mortality ; Cadherins/metabolism* ; Carcinoma, Ductal, Breast/drug therapy ; Carcinoma, Ductal, Breast/metabolism ; Carcinoma, Ductal, Breast/mortality ; Carcinoma, Medullary/drug therapy ; Carcinoma, Medullary/metabolism ; Carcinoma, Medullary/mortality ; Cell Death/drug effects ; Female ; Humans ; Ki-67 Antigen/metabolism ; Middle Aged ; Predictive Value of Tests ; Proto-Oncogene Proteins c-bcl-2/metabolism ; Receptors, Androgen/metabolism* ; Tumor Suppressor Protein p53/metabolism

Keywords: androgen receptor ; breast cancer ; chemosensitivity ; E-cadherin ; triple negative

Abstract: OBJECTIVE: The purpose of this study was to evaluate the impact of various pathologic and biologic factors in triple-negative breast cancer (TNBC) on chemotherapy response using in vitro ATP-based chemotherapy response assay (ATP-CRA).

METHODS: Forty-seven cases of TNBC were included. Immunohistochemical stains for androgen receptor (AR), p53, CD10, c-kit, CK5/6, vimentin, bcl-2, E-cadherin, Ki-67 and epidermal growth factor receptor were performed. In vitro ATP-CRA was used to analyze chemosensitivity for 5-fluorouracil (5-FU), docetaxel, doxorubicin, epirubicin, vinorelbine, gemcitabine, methotrexate (MTX), oxaliplatin and paclitaxel.

RESULTS: The results showed that all cytotoxic agents demonstrated the trend that E-cadherin-expressing cases had a higher cell death rate than E-cadherin-negative cases. Particularly, vinorelbine showed statistical significance (P = 0.004). Cases with AR expression showed higher cell death rates than those without in 5-FU and MTX (P = 0.012 and 0.014, respectively).

CONCLUSIONS: E-cadherin and AR could be candidate predictive factors for chemotherapy response in TNBC. Further in vivo study is required to clarify their roles.