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Role of pancreatitis-associated protein 1 on oxidative stress-induced cell death of pancreatic acinar cells

Authors
 Joo Weon Lim  ;  Ji Yeon Song  ;  Jeong Yeon Seo  ;  Hyeyoung Kim  ;  Kyung Hwan Kim 
Citation
 ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, Vol.1171 : 545-548, 2009 
Journal Title
 ANNALS OF THE NEW YORK ACADEMY OF SCIENCES 
ISSN
 0077-8923 
Issue Date
2009
MeSH
Animals ; Antigens, Neoplasm/genetics ; Antigens, Neoplasm/physiology* ; Apoptosis/physiology* ; Biomarkers, Tumor/genetics ; Biomarkers, Tumor/physiology* ; Cell Line, Tumor ; Cell Survival/physiology ; DNA, Antisense/genetics ; DNA, Complementary/genetics ; Gene Expression Regulation, Neoplastic ; Lectins, C-Type/genetics ; Lectins, C-Type/physiology* ; Oxidative Stress/physiology* ; Pancreatic Neoplasms/genetics ; Pancreatic Neoplasms/pathology ; Pancreatitis-Associated Proteins ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Rats ; Reverse Transcriptase Polymerase Chain Reaction ; Transfection
Keywords
pancreatitis-associated protein 1 ; cell death ; pancreatic acinar cells
Abstract
Previously we showed that oxidative stress induces apoptosis of pancreatic acinar cells with nuclear loss of DNA repair proteins. Oxidative stress has an important role in the pathogenesis of pancreatitis. Pancreatitis-associated protein 1 (PAP-1) is a protein secreted upon induction of acute pancreatitis. In this study, we investigated the role of PAP-1 on oxidative stress-induced cell death of pancreatic acinar AR42J cells. AR42J cells were transfected with or without full-length sense cDNA of PAP-1 (PAP-1 S cDNA) or antisense cDNA of PAP-1 (PAP-1 AS cDNA) and received oxidative stress caused by glucose oxidase acting on beta-D-glucose, glucose/glucose oxidase. PAP-1 mRNA expression and cell viability were determined. As a result, oxidative stress induced PAP-1 mRNA expression in AR42J cells in a time-dependent manner. Cell viability decreased with the concentration of glucose oxides delivered to the cells that had received glucose. Oxidative stress-induced PAP-1 expression was augmented in the cells transfected with PAP-1 S cDNA compared with wild-type cells or cells transfected with control vector pcDNA. PAP-1 induction by oxidative stress decreased in the cells transfected with PAP-1 AS cDNA. Cell death caused by oxidative stress was inhibited in the cells transfected with PAP-1 S cDNA, but it increased in the cells transfected with PAP-1 AS cDNA. These results indicate that PAP-1 may be a defensive gene for oxidative stress-induced cell death of pancreatic acinar cells
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/j.1749-6632.2009.04702.x/abstract
DOI
10.1111/j.1749-6632.2009.04702.x
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Hwan(김경환)
Song, Ji Yeon(송지연)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/104339
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