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Zonal responsiveness of the human intervertebral disc to bone morphogenetic protein-2.

 Hyang Kim  ;  Jeong-Uk Lee  ;  Seong-Hwan Moon  ;  Hyung-Chan Kim  ;  Un-Hae Kwon  ;  Nam-Hun Seol  ;  Ho-Joong Kim  ;  Jin-Oh Park  ;  Heoung-Jae Chun  ;  Il-Keun Kwon  ;  Hwan-Mo Lee 
 SPINE, Vol.34(17) : 1834-1838, 2009 
Journal Title
Issue Date
Adult ; Bone Morphogenetic Protein 2/metabolism ; Bone Morphogenetic Protein 2/pharmacology* ; Bone Morphogenetic Protein 2/therapeutic use ; Cells, Cultured ; Chondrocytes/drug effects ; Chondrocytes/metabolism ; Collagen Type I/genetics ; DNA/biosynthesis ; DNA/drug effects ; Extracellular Matrix/drug effects ; Extracellular Matrix/metabolism ; Homeodomain Proteins/genetics ; Humans ; Intervertebral Disc/drug effects* ; Intervertebral Disc/metabolism* ; Intervertebral Disc/physiopathology ; Intervertebral Disc Displacement/drug therapy* ; Intervertebral Disc Displacement/metabolism* ; Intervertebral Disc Displacement/physiopathology ; Middle Aged ; Osteocalcin/genetics ; Osteopontin/genetics ; Proteoglycans/drug effects ; Proteoglycans/genetics ; Proteoglycans/metabolism ; RNA, Messenger/drug effects ; RNA, Messenger/metabolism ; Regeneration/drug effects* ; Regeneration/physiology ; Transcription Factors/genetics ; Up-Regulation/drug effects ; Up-Regulation/genetics
bone morphogenetic protein-2 ; intervertebral disc cell ; proteoglycan synthesis ; zonal effect
STUDY DESIGN: In vitro experiment using bone morphogenetic protein-2 (BMP-2) and cells from the nucleus pulposus (NP), transitional zone (TZ), and anulus fibrosus (AF) of the human intervertebral disc (IVD). OBJECTIVE: To demonstrate the differential effect of BMP-2 on DNA synthesis, proteoglycan synthesis, and osteocalcin mRNA expression in human IVD cells from the NP, TZ, and AF, respectively. SUMMARY OF BACKGROUND DATA: BMP-2 has been proven to be effective in stimulating proteoglycan synthesis in articular chondrocytes and IVD cells from the NP. Nevertheless, the effect of BMP-2 on cells from different regions of the IVD has not yet been thoroughly elucidated. METHODS: Human IVDs were harvested from surgical disc procedures and tissue from the NP, TZ, and AF was obtained. Disc tissue was enzymatically digested, and IVD cells were cultured three-dimensionally in alginate beads. Then IVD cell cultures from the NP, TZ, and AF were exposed to BMP-2. DNA synthesis and newly synthesized proteoglycan were measured. Reverse transcription-polymerase chain reaction for mRNA expression of bone sialoprotein, DLX5, osteocalcin, and collagen type I, was performed. RESULTS: Cells from the AF responded to BMP-2 with mitogenesis. There was no significant increase in DNA synthesis in cultures from the NP and TZ treated with BMP-2. Only cells from the NP showed a significant increase in newly synthesized proteoglycan in response to BMP-2. IVD cells from all zones demonstrated no significant expression of bone sialoprotein, DLX5, osteocalcin mRNA after treatment with BMP-2. CONCLUSION: BMP-2 clearly exerted a mitogenic effect on AF cells, and stimulated proteoglycan synthesis in NP cells. However, BMP-2 did not have an osteogenic effect in any IVD region. Taken together, these results confirm that BMP-2 can be used as an anabolic agent for mitogenesis in AF cells and NP cell matrix regeneration without the possibility of osteogenesis
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1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
Yonsei Authors
Kwon, Un Hye(권언혜)
Kim, Hyang(김향)
Kim, Hyung Chan(김형찬)
Kim, Ho Joong(김호중)
Moon, Seong Hwan(문성환)
Park, Jin Oh(박진오)
Lee, Hwan Mo(이환모) ORCID logo https://orcid.org/0000-0002-5405-3832
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