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[6]-Gingerol suppresses interleukin-1 beta-induced MUC5AC gene expression in human airway epithelial cells.

Authors
 Jeong Hong Kim  ;  Jung Hyun Chang  ;  Joo-Heon Yoon  ;  Soon Ho Kwon  ;  Jung Ho Bae  ;  Kyung-Su Kim 
Citation
 AMERICAN JOURNAL OF RHINOLOGY & ALLERGY, Vol.23(4) : 385-391, 2009 
Journal Title
AMERICAN JOURNAL OF RHINOLOGY & ALLERGY
ISSN
 1945-8924 
Issue Date
2009
MeSH
Blotting, Western ; Catechols/pharmacology* ; Cell Proliferation/drug effects ; Cells, Cultured ; Extracellular Signal-Regulated MAP Kinases/pharmacology ; Fatty Alcohols/pharmacology* ; Gene Expression Regulation/drug effects* ; Ginger ; Humans ; Interleukin-1beta/pharmacology* ; Mucin 5AC/biosynthesis ; Mucin 5AC/drug effects ; Mucin 5AC/genetics* ; RNA, Messenger/biosynthesis ; RNA, Messenger/drug effects ; RNA, Messenger/genetics* ; Respiratory Mucosa/cytology ; Respiratory Mucosa/drug effects ; Respiratory Mucosa/metabolism* ; Reverse Transcriptase Polymerase Chain Reaction ; Signal Transduction/drug effects ; p38 Mitogen-Activated Protein Kinases/pharmacology
Keywords
Antihypersecretory agent ; extracellular signal-regulated kinase ; [6]-gingerol ; human airway epithelial cell ; inter-leukin-1 beta ; p38 mitogen-activated protein kinase ; MUC5AC
Abstract
BACKGROUND: [6]-Gingerol is a major active component of ginger and a natural polyphenol compound. The present study investigated whether [6]-gingerol suppresses interleukin (IL)-1 beta-induced MUC5AC gene expression in human airway epithelial cells and, if so, examined whether the suppression of MUC5AC gene expression is mediated via the mitogen-activated protein kinase (MAPK) signal transduction pathway.

METHODS: MUC5AC mRNA and protein were measured using reverse transcription-polymerase chain reaction (PCR), real-time PCR, and Western blot analysis in cultured NCI-H292 human airway epithelial cells. Extracellular signal-regulated kinase (ERK) and p38 MAPK protein levels were analyzed by Western blot.

RESULTS: Expression of MUC5AC mRNA increased in NCI-H292 cells upon treatment with 10 ng/mL of IL-1 beta for 24 hours. When the cells were pretreated with 10 microM of [6]-gingerol, expression of IL-1 beta-induced MUC5AC mRNA and protein was significantly suppressed. Suppression of IL-1 beta-induced MUC5AC mRNA was also observed in cells pretreated with ERK- or p38 MAPK-specific inhibitors, suggesting that [6]-gingerol-mediated suppression of IL-1 beta-induced MUC5AC mRNA operated via the ERK- and p38 MAPK-dependent pathways.

CONCLUSIONS: [6]-Gingerol suppresses IL-1 beta -induced MUC5AC gene expression in human airway epithelial cells via the ERK- and p38 MAPK-dependent pathways; therefore, [6]-gingerol may be considered a possible anti-hypersecretory agent.
Full Text
http://www.ingentaconnect.com/content/ocean/ajra/2009/00000023/00000004/art00004?token=004e15f7536a141333c4a2f7a7a6a6f572b6756767034254f6d622244502c6876305021180142c
DOI
10.2500/ajra.2009.23.3337
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kwon, Soon Ho(권순호)
Kim, Kyung Su(김경수) ORCID logo https://orcid.org/0000-0003-1460-0640
Yoon, Joo Heon(윤주헌)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/104168
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