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Subtelomeric DNA methylation and telomere length in human cancer cells

Authors
 Myung Eun Lee  ;  Sun Young Rha  ;  Hei-Cheul Jeung  ;  Hyun Cheol Chung  ;  Bong-Kyeong Oh 
Citation
 CANCER LETTERS, Vol.281(1) : 82-91, 2009 
Journal Title
CANCER LETTERS
ISSN
 0304-3835 
Issue Date
2009
MeSH
Cell Line, Tumor/chemistry ; Cell Line, Tumor/ultrastructure ; Chromosomes, Human/chemistry ; Chromosomes, Human/ultrastructure ; DNA Methylation* ; DNA, Neoplasm/chemistry ; DNA, Neoplasm/genetics* ; DNA, Neoplasm/ultrastructure ; Heterochromatin/chemistry ; Heterochromatin/ultrastructure ; Humans ; Neoplasms/chemistry ; Neoplasms/genetics* ; Neoplasms/ultrastructure ; Polymerase Chain Reaction ; Sequence Analysis, DNA ; Sulfites/pharmacology ; Telomere/ultrastructure*
Keywords
Subtelomere ; DNA methylation ; Telomere ; Heterochromatin ; Cancer cell
Abstract
Subtelomeric epigenetic modifications are known to be associated with telomere length. We examined subtelomeric DNA methylation at seven sites for five chromosomes by methylation-specific PCR (MSP) and two sites for two chromosomes by bisulfite genomic sequencing (BGS) in 20 human cancer cell lines and subsequently analyzed their association with telomere length. Full-methylation (55/140) was more frequently found compared to un-methylation (35/140) (p=0.01). Subtelomeric-methylation patterns varied from region to region; full-methylation and un-methylation were dominant at one of 9q sites (20/20) and 9p (18/20), respectively. MSP and BGS data exhibited no apparent correlation between methylation status and telomere length. In addition, Hep3B subclones that possessed different telomere lengths exhibited no changes in methylation status according to telomeres. In summary, subtelomeres might form distinct chromatin structures from region to region and effect of subtelomeric DNA methylation on telomere regulation might be little.
Full Text
http://www.sciencedirect.com/science/article/pii/S0304383509001244
DOI
10.1016/j.canlet.2009.02.031
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Rha, Sun Young(라선영) ORCID logo https://orcid.org/0000-0002-2512-4531
Chung, Hyun Cheol(정현철) ORCID logo https://orcid.org/0000-0002-0920-9471
Jeung, Hei Cheul(정희철) ORCID logo https://orcid.org/0000-0003-0952-3679
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/104046
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