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J.Leonard Goldner Award 2008. Effect of extracorporeal shock wave therapy on cultured tenocytes

Authors
 Seung Hwan Han  ;  Jin Woo Lee  ;  Gregory P. Guyton  ;  Brent G. Parks  ;  Jean-Paul Courneya  ;  Lew C. Schon 
Citation
 FOOT & ANKLE INTERNATIONAL, Vol.30(2) : 93-98, 2009 
Journal Title
 FOOT & ANKLE INTERNATIONAL 
ISSN
 1071-1007 
Issue Date
2009
MeSH
Awards and Prizes* ; Cells, Cultured ; Cytokines/metabolism ; High-Energy Shock Waves/therapeutic use* ; Humans ; Matrix Metalloproteinases/metabolism ; Tendinopathy/metabolism ; Tendons/cytology* ; Tendons/metabolism ; Tendons/radiation effects*
Keywords
Extracorporeal Shock Wave ; Tendon ; Cells ; ESWT
Abstract
BACKGROUND: Little has been reported about the biologic effect of shock waves on human normal or pathologic tendon tissue. We hypothesized that inflammatory cytokine and MMP production would be down-regulated by shock wave stimulation. MATERIALS AND METHODS: Diseased Achilles tendon tissue and healthy flexor hallucis longus tissue were used. Shock wave treatment was applied to cultured cells at 0.17 mJ/mm(2)energy 250, 500, 1000, and 2000 times. RESULTS: A dose-dependent decrease in cell viability was noted in cells receiving 1000 and 2000 shocks (86.0 +/- 5.6%, p = 0.01 and 72.4 +/- 8.9%, p = 0.001) as compared with the normal control. Cell count in the 500-shock group increased by 23.4% as compared with the control (p = 0.05). The concentration of MMP 1, 2, and 13 was higher in diseased tenocytes as compared with normal cells (p = 0.04, all comparisons). IL-6 levels were higher in the diseased tenocytes as compared with normal tenocytes (44.10 +/- 16.72 versus 0.21 +/- 0.55 ng/ml, (p < 0.05). IL-1 levels in normal cells increased (2.24 +/- 5.02 ng/ml to 9.31 +/- 6.85 ng/ml) after shock wave treatment (p = 0.04). In diseased tenocytes, levels of MMP-1 (1.12 +/- 0.23 to 0.75 +/- 0.24 ng/ml; p = 0.04) and MMP-13 (1.43 +/- 0.11 to 0.80 +/- 0.15 ng/ml; p = 0.04) were significantly decreased after shock wave treatment. The IL-6 level in diseased tenocytes was decreased (44.10 +/- 16.72 to 14.66 +/- 9.49 ng/ml) after shock wave treatment (p = 0.04). CONCLUSION: Higher levels of MMPs and ILs were found in human tendinopathy-affected tenocytes as compared with normal cells. ESWT decreased the expression of several MMPs and ILs. CLINICAL RELEVANCE: This mechanism may play an important role in shock wave treatment of tendinopathy clinically
Full Text
http://fai.sagepub.com/content/30/2/93.abstract
DOI
10.3113/FAI.2009.0093
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
Yonsei Authors
Lee, Jin Woo(이진우) ORCID logo https://orcid.org/0000-0002-0293-9017
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/103919
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