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Characteristics of B-cell-specific growth substance produced by Bacillus licheniformis E1

Authors
 Kim, Joo Young  ;  Kun Sub Chung  ;  Jeon Han Park  ;  Yi-Sub Kwak  ;  Bong Ki Lee 
Citation
 JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, Vol.19(1) : 55-64, 2009 
Journal Title
 JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY 
ISSN
 1017-7825 
Issue Date
2009
MeSH
Adjuvants, Immunologic/analysis ; Animals ; Anti-Bacterial Agents/pharmacology ; B-Lymphocytes/cytology* ; B-Lymphocytes/drug effects ; Bacillus/metabolism* ; Cell Culture Techniques ; Female ; Growth Substances/pharmacology ; Growth Substances/physiology* ; Immunoglobulin G ; Immunoglobulin M ; Lipopolysaccharides/antagonists & inhibitors ; Lipopolysaccharides/physiology ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C3H ; Polymyxin B/pharmacology ; Precursor Cells, B-Lymphoid/cytology ; Precursor Cells, B-Lymphoid/drug effects ; T-Lymphocytes/cytology ; T-Lymphocytes/drug effects ; Toll-Like Receptor 4/metabolism
Keywords
B cellspecific growth substance (BGS) ; Adjuvant ; Bacillus licheniformis E1 ; immunomodulator
Abstract
A B cell-specific growth substance (BGS) was isolated from the slime layer of Bacillus licheniformis E1. Unlike LPS, the BGS was not affected by polymixin B, an inhibitor of LPS, or by TLR4, and resulted in the growth of B cells. When BALB/c mice were treated with the BGS, the B cell population was found to increase in both the bone marrow and the spleen, with a marked increase after 24 h in the bone marrow and after 48 h in the spleen. When using antibodies to B cell lineage-restricted surface molecules to analyze the B cell population changes resulting from treatment with the BGS, an increase in immature B cells (IgM(+) and AA4.1(+)) and mature B cells (IgM(+) and IgD(+)) was found in the bone marrow 24 h after treatment with the BGS, whereas a decrease in mature B cells and increase in IgG(+) B cells were found in the spleen. When the BGS and OVA antigen were injected into the peritoneal cavity of BALB/c mice, this resulted in a high OVA-specific antibody titer in the sera, similar to that induced by aluminum hydroxide. Therefore, it is anticipated that the mass production of the BGS by B. licheniformis E1 could be used for studies of B cells in immunology, and contribute to the development of a new adjuvant for vaccine manufacture.
Files in This Item:
T200900983.pdf Download
DOI
10.4014/jmb.0806.396
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Joo Young(김주영)
Park, Jeon Han(박전한) ORCID logo https://orcid.org/0000-0001-9604-3205
Lee, Bong Ki(이봉기)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/103744
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