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hnRNP L binds to CA repeats in the 3'UTR of bcl-2 mRNA

Authors
 Dong-Hyoung Lee  ;  Mi-Hyun Lim  ;  Dong-Ye Youn  ;  Seung Eun Jung  ;  Young Soo Ahn  ;  Yoshihide Tsujimoto  ;  Jeong-Hwa Lee 
Citation
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.382(3) : 583-587, 2009 
Journal Title
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 
ISSN
 0006-291X 
Issue Date
2009
MeSH
3' Untranslated Regions/genetics ; 3' Untranslated Regions/metabolism* ; Cell Line, Tumor ; Heterogeneous-Nuclear Ribonucleoprotein L/genetics ; Heterogeneous-Nuclear Ribonucleoprotein L/metabolism* ; Humans ; Proto-Oncogene Proteins c-bcl-2/genetics* ; RNA Stability* ; Repetitive Sequences, Nucleic Acid*
Keywords
bcl-2 ; mRNA stability ; hnRNP L ; CA repeats
Abstract
We previously reported that the CA-repeat sequence in the 3'-untranslated region (3'UTR) of bcl-2 mRNA is involved in the decay of bcl-2 mRNA. However, the trans-acting factor for the CA element in bcl-2 mRNA remains unidentified. the heterogeneous nuclear ribonucleoprotein L (hnRNP L), an intron splicing factor, has been reported to bind to CA repeats and CA clusters in the 3'UTR of several genes. We reported herein that the CA repeats of bcl-2 mRNA have the potential to form a distinct ribonuclear protein complex in cytoplasmic extracts of MCF-7 cells, as evidenced by RNA electrophoretic mobility shift assays (REMSA). A super-shift assay using the hnRNP L antibody completely shifted the complex. Immunoprecipitation with the hnRNP L antibody and MCF-7 cells followed by RT-PCR revealed that hnRNP L interacts with endogenous bcl-2 mRNA in vivo. Furthermore, the suppression of hnRNP L in MCF-7 cells by the transfection of siRNA for hnRNP L resulted in a delay in the degradation of RNA transcripts including CA repeats of bcl-2 mRNA in vitro, suggesting that the interaction between hnRNPL and CA repeats of bcl-2 mRNA participates in destabilizing bcl-2 mRNA.
Full Text
http://www.sciencedirect.com/science/article/pii/S0006291X09005373
DOI
10.1016/j.bbrc.2009.03.069
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
Yonsei Authors
Ahn, Young Soo(안영수)
Lee, Dong Hyoung(이동형)
Jung, Seung Eun(정승은)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/103708
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