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조리방법에 따른 참깨의 알레르기 항원성 비교

Other Titles
 Differences between Raw and Variously Cooked Sesame Seeds on the Allergenicity 
Authors
 이경은  ;  홍정연  ;  손선미  ;  박선희  ;  이상일  ;  손명현  ;  김규언  ;  김경원 
Citation
 Pediatric Allergy and Respiratory Disease (소아알레르기 호흡기학회지), Vol.19(1) : 56-62, 2009 
Journal Title
 Pediatric Allergy and Respiratory Disease (소아알레르기 호흡기학회지) 
ISSN
 1225-679X 
Issue Date
2009
Keywords
Sesame ; Allergen ; Allergenicity ; Immunoglobulin E
Abstract
Purpose: There has been a significant increase in the number on reports of hypersensitivity to sesame, probably because of its use in international fast-food and bakery products. Thus, we have investigated whether various cooking methods affect the allergenicity of sesame. Methods: Sesame seeds were roasted, boiled or fried with vegetable oil, and then their proteins were each extracted. The proteins were separated by sodium dodecyl sulfate-poly-acrylamide gel electrophoresis (SDS-PAGE) and detection of immunoglobulin (Ig) E specific to sesame seed proteins was performed with Western blotting using 6 sera. Results: SDS-PAGE of raw sesame proteins showed various-sized bands including 7, 9, 12, 15 and 17 kD known as major allergens. While only few protein bands remained in roasted or fried sesame seeds, some protein bands sized under 15 kD were observed in the boiled sesame seeds and their soup. The pooled sera yielded IgE-specific reaction with 7 kD in raw and boiled, and 37 kD in fried and boiled sesame proteins. Conclusion: These findings suggest that the cooking methods may change the allergenicity of sesame seed proteins.
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Won(김경원) ORCID logo https://orcid.org/0000-0003-4529-6135
Kim, Kyu Earn(김규언)
Sohn, Myung Hyun(손명현) ORCID logo https://orcid.org/0000-0002-2478-487X
Son, Sun Mi(손선미)
Hong, Jung Yeon(홍정연) ORCID logo https://orcid.org/0000-0003-0406-9956
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/103655
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